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Soluble elastin

Manufactured by Merck Group
Sourced in United States, Italy

Soluble elastin is a laboratory-grade protein derived from natural elastin. It is a purified and soluble form of the extracellular matrix protein elastin. Soluble elastin can be used in various research applications, including cell culture studies and biomaterial development.

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2 protocols using soluble elastin

1

Elastin-Based Immunomodulation Assay

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Monoclonal anti-elastin antibody BA4, IgG, soluble elastin, and LPS were purchased from Sigma-Aldrich (St. Louis, MO, USA). VGVAPG peptide was purchased from Elastin Products Company (Owensville, MO, USA). Mouse IFN-γ and IL-4 were purchased from R&D systems (Minneapolis, MN, USA).
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2

Biomimetic Hydrogel Fabrication

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Gelatin (type B from bovine skin), gellan, soluble elastin from bovine neck ligament, 1-(3-dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride (EDC), N-hydroxysuccinimide (NHS), and phosphate buffered saline (PBS) were supplied by Sigma-Aldrich (Milan, Italy). The peptide sequences Gly-Gly-Gly-Arg-Glu-Asp-Val (GGGREDV) and Gly-Gly-Gly-Gly-Arg-Gly-Asp-Ser-Pro (GGGGRGDSP) were synthesized and provided by Cambridge Research Biochemicals (Billingham, Cleveland, UK). Moreover, calcium chloride and acetone were purchased from Carlo Erba Reagenti (Milan, Italy). All the other reagents were commercially available and used as received.
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