Culture methods and target cells are summarized in Table 1 . BEAS-2B (No. CRL-9609; ATCC, Manassas, VA) immortalized human lung epithelial cells were cultured in BEGM media with a Single Quot supplement kit (No. CC-3170; Lonza, Basel, Switzerland) at 37°C in 5% CO2. LNCaP (No. CRL-1740; ATCC) human prostate adenocarcinoma cells were cultured using phenol-red free RPMI-1640 (No. 11835; Life Technologies, Grand Island, NY) with 10% fetal bovine serum (FBS) and penicillin/streptomycin at 37°C in 5% CO2. PLHC-1 (No. CRL-2406; ATCC) fish (Poeciliopsis lucida) hepatoma cells were cultured using EMEM media (No. 30–2003; ATCC) with 5% FBS and penicillin/streptomycin at 30°C in 5% CO2.
Plhc 1
Manufactured by American Type Culture Collection
Sourced in United States
The PLHC-1 is a cell line derived from the Pimephales promelas (fathead minnow) that exhibits epithelial-like morphology. It is maintained in culture and can be used for various research applications.
Automatically generated - may contain errors
Lab products found in correlation
Hep3b, by American Type Culture Collection (4 mentions)
Penicillin, by Thermo Fisher Scientific (4 mentions)
Streptomycin, by Thermo Fisher Scientific (4 mentions)
Hepg2, by American Type Culture Collection (3 mentions)
Snu398, by American Type Culture Collection (2 mentions)
Thle 3, by American Type Culture Collection (2 mentions)
Phenol red free rpmi 1640, by Thermo Fisher Scientific (1 mentions)
Beas 2b, by American Type Culture Collection (1 mentions)
Single quot supplement kit, by Lonza (1 mentions)
Lncap, by American Type Culture Collection (1 mentions)
Emem media, by American Type Culture Collection (1 mentions)
Snu 449, by American Type Culture Collection (1 mentions)
Plc prf 5, by American Type Culture Collection (1 mentions)
Sk hep1, by American Type Culture Collection (1 mentions)
Idelalisib, by Selleck Chemicals (1 mentions)
Sorafenib, by Selleck Chemicals (1 mentions)
Huh7 cell line, by Japanese Collection of Research Bioresources Cell Bank (1 mentions)
Doxorubicin, by Selleck Chemicals (1 mentions)
Lipofectamine 2000, by Thermo Fisher Scientific (1 mentions)
Fetal bovine serum (fbs), by Thermo Fisher Scientific (1 mentions)
6 protocols using plhc 1
1
Culture Methods for Cell Lines
2
Maintaining PLHC-1 and ZFL Cell Lines
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Cell lines PLHC-1 (ATCC CRL-2406) and
ZFL (ATCC CRL-2643) were maintained in 75 cm2 flasks at
28 °C in a 5% CO2-humidified atmosphere. PLHC-1 cells
were grown in EMEM containing 0.1 mM nonessential amino acids (l -alanine, l -asparagine, l -aspartic acid, l -glutamic acid, glycine, l -proline, L-serine), supplemented
with 5% FBS. ZFL cells were grown in a mixture of L-15, DMEM, and
F-12 (50%–35%–15%) supplemented with 5% fetal bovine
serum (FBS), 0.5% rainbow trout serum, 0.01 mg/mL insulin, and 50
ng/mL epidermal growth factor. Both cell lines were maintained in
the presence of penicillin–streptomycin (50 000 U/L
to 50 mg/L). The medium was changed every 2 days, and cells were passed
once a week by trypsinization (0.05% trypsin–0.53 mM EDTA).
ZFL and PLHC-1 cells were plated at a density of 1.33 × 104 and 2.00 × 104 cells/cm2, respectively.
PLHC-1 and ZFL cells were maintained for 10 and 6 days, respectively,
to ensure the confluence.
ZFL (ATCC CRL-2643) were maintained in 75 cm2 flasks at
28 °C in a 5% CO2-humidified atmosphere. PLHC-1 cells
were grown in EMEM containing 0.1 mM nonessential amino acids (
with 5% FBS. ZFL cells were grown in a mixture of L-15, DMEM, and
F-12 (50%–35%–15%) supplemented with 5% fetal bovine
serum (FBS), 0.5% rainbow trout serum, 0.01 mg/mL insulin, and 50
ng/mL epidermal growth factor. Both cell lines were maintained in
the presence of penicillin–streptomycin (50 000 U/L
to 50 mg/L). The medium was changed every 2 days, and cells were passed
once a week by trypsinization (0.05% trypsin–0.53 mM EDTA).
ZFL and PLHC-1 cells were plated at a density of 1.33 × 104 and 2.00 × 104 cells/cm2, respectively.
PLHC-1 and ZFL cells were maintained for 10 and 6 days, respectively,
to ensure the confluence.
3
Culturing Human Hepatocellular Carcinoma Cell Lines
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The cell lines of human HCC, including HepG2 (ATCC® HB-8065TM), Hep3B (ATCC® HB-8064TM), SNU-449 (ATCC® CRL-2234TM), PLC/PRF/5 (ATCC® CRL-8024TM), SK-HEP-1 (ATCC® HTB-52TM), and PLHC-1 (ATCC® CRL-2406TM), were purchased from ATCC (American Type Culture Collection), and then cultured in DMEM with 15% FBS accompanied by Streptomycin and Penicillin (bi-antibiotics) with atmosphere (5% CO2) at 37 °C.
4
Cell Line Culture Protocols
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The hepatocellular carcinoma cell lines (PLHC1, Hep3B, and SNU398), a hepatoblastoma cell line (HepG2) and a human hepatocyte cell line (THLE3) were obtained from the American Type Culture Collection and had been authenticated by short tandem repeat profiling. Dulbecco’s modified Eagle medium (DMEM; Invitrogen; Thermo Fisher Scientific, Inc.) containing 10% fetal bovine serum (FBS), 100 U/mL penicillin, and 100 μg/mL streptomycin (Invitrogen; Thermo Fisher Scientific, Inc.) was used to culture all cells at 37°C in 5% CO2.
5
Establishment and Characterization of HCC Cell Lines
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The HCC cell lines, Hep G2, Hep3B, SNU-398, PLHC-1 and embryonic kidney 293T cells were obtained from American Type Culture Collection (ATCC, Manassas, VA, USA). HuH-7 cell line was got from Japanese Collection of Research Bioresources (JCRB), normal hepatocyte cell line HL-7702 was got from Institute of Biochemistry and Cell Biology (SIBS, CAS, Shanghai, China). All HCC cells were cultured in DMEM medium supplemented with 10% fetal bovine serum (FBS), 100 µg/mL streptomycin and 100 units/mL penicillin (Invitrogen, Carlsbad, CA, USA). The drugs, including idelalisib, sorafenib, doxorubicin (Selleckchem, Houston, TX, USA), were diluted with DMSO. Constitutively active AKT was obtained from Addgene (Cambridge, MA, USA).
6
HCC Cell Lines and Transfection Methods
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Human HCC cell lines (Hep3B, HepG2, SK-help1, and PLHC-1) and human normal hepatocyte cell line (THLE-3) were procured from the American Type Culture Collection (ATCC, Manassas, VA, USA). All cells were cultured in RPMI-1640 medium (Gibco, Carlsbad, CA, USA) containing 10% fetal bovine serum (FBS) (Gibco, Carlsbad, CA, USA) and 100 U/mL penicillin, and 100 μg/mL streptomycin (Invitrogen, Carlsbad, CA, USA) at 37°C with 5% CO2. pcDNA3.1-UBE2T, empty vector, UBE2T siRNA (si-UBE2T#1/si-UBE2T#2), scramble siRNA, miR-212-5p mimics, miR-212-5p inhibitors and negative controls (mimics NC/inhibitors NC) were available from Invitrogen (Carlsbad, CA, USA). Hep3B and HepG2 cells were transfected with Lipofectamine® 2000 (Invitrogen, Carlsbad, CA, USA) according to the manufacturer’s instructions.
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