Petroleum ether

Manufactured by Beijing Chemical Works

Sourced in China

Petroleum ether is a clear, volatile liquid distilled from petroleum. It is commonly used as a solvent in chemical laboratories for the extraction and purification of various organic compounds.

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Lab products found in correlation

11 protocols using petroleum ether

Determination of PUFA Composition in Oviductus Ranae

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Petroleum ether, methanol, and phosphoric acid were analytical pure and purchased from Beijing Chemical Factory (Beijing, China). HPLC-grade acetonitrile and HPLC-grade methanol were purchased from Fisher (Fisher Scientific, Fair Lawn, NJ, USA). The ultrapure water was prepared from a gradient water purification system (Water Purifier, Sichuan, China). Eicosapentaenoic acid (EPA, Lot No: 32510020), docosahexaenoic acid (DHA, Lot No: 0501808), and arachidonic acid (ARA, Lot No: 2491801) were purchased from TanMo Quality Testing Technology Co., Ltd. (Beijing, China). α-Linolenic acid (ALA, Lot No: U-62A-J25-C), linoleic acid (LA, Lot No: U-52A-JA3-C), and oleic acid (OA, Lot No: U-46A-MA6-C) were purchased from ANPEL Laboratory Technologies (Shanghai) Inc. (Shanghai, China). The purity of each standard compound was higher than 99%, as determined by HPLC. Six kinds of PUFAs standards were formulated to different concentrations using HPLC-grade methanol and filtrated with a 0.22 μm filter membrane prior HPLC injection.
Jilin Province Rana Industry Association provided a total of 27 samples of Oviductus Ranae, which were collected from the Changbai Mountains area in Jilin Province, China. Collection sources covered the major production areas of Changbai Mountains. The specific collection information is shown in Table 1, and Figure 2 shows the geographical distribution of the samples.

Guo H., Gan Y., Liu M., Wang S., Ni S., Zhou Y., Xiao Y., Wang Z, & Wang Y. (2019). Quality Evaluation of Oviductus Ranae Based on PUFAs Using HPLC Fingerprint Techniques Combined with Chemometric Methods. Foods, 8(8), 322.

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Synthesis of Functionalized Polymeric Nanocarriers

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Pluronic F127 and methane sulfonyl chloride were obtained from Sigma-Aldrich. N_ε-Benzyloxycarbonyl-l-lysine (l-lysine(z)) was purchased from Energy Chemical. Polyethylene glycol (PEG4000), triethylamine, triphosgene, trifluoroacetic acid (TFA), 5-FU (98%), and trifluoroacetic acid-d (TFA-d) were obtained from Aladdin. Moreover, 33% HBr/acetic acid solution was acquired from J&K Chemical. Dichloromethane, tetrahydrofuran (THF), N,N-dimethylformamide (DMF), chloroform, petroleum ether, ammonium hydroxide solution, potassium dihydrogen phosphate, and sodium hydroxide were purchased from Beijing Chemical Works. Dimethyl sulfoxide-d₆ (DMSO-d₆) was obtained from CIL.

Li P., Dai X., Qu L., Sui Y, & Zhang C. (2020). Dual responsive oligo(lysine)-modified Pluronic F127 hydrogels for drug release of 5-fluorouracil. RSC Advances, 10(41), 24507-24514.

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Analytical Methods for Soybean Oil and Potato Chips

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Soybean oil was purchased from Zhongliang Eastocean Oils & Grains Industries (Zhangjiagang) Company, Limited. Quick‐frozen potato chips were supplied by Shanghai Sheng Fang Industrial Company, Limited. Sodium hydroxide, ether (absolute), isopropyl alcohol, ethanol, trichloroacetic acid, benzene, potassium hydroxide, and petroleum ether were obtained from Beijing Chemical Works. Phenolphthalein was provided by Tianjin Beichen Fangzheng Reagent Factory. 2,4‐Dinitrophenylhydrazine was obtained from Tianjin Ruijin Chemical Company, Limited.
HH‐S digital display constant temperature oil bath was supplied by Changzhou Rong Hua Instrument Manufacturing Company, Limited. HH‐ZK1 constant temperature control was obtained from Gongyi Yuhua Instrument Liability Company, Limited. The spherical condensation tube and glass dryer were supplied by Beijing Huabo Station Biological Analysis Technology Company, Limited. UV‐9000S double beam UV–vis spectrophotometer was obtained from Shanghai Yuanshan Instrument Company, Limited. The YZF vacuum dryer was purchased from Shanghai Yao Instrument Equipment Factory.

Li Y., Cao X., Cao Y., Feng Y., Ji J., Xie J, & Wang X. (2020). Physicochemical parameter based estimation of discarding points for frying oil using data interpolation and principal component transformation. Food Science & Nutrition, 8(5), 2360-2372.

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NMR and MS Analysis of Organic Compounds

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NMR spectra were recorded on a Bruker Avance III 600 spectrometer (Bruker, Billerica, Germany) with ¹H-NMR at 600 MHz and ¹³C-NMR at 150 MHz, with chemical shift values expressed as δ values, using deuterated solvent signals as an internal reference. HR-ESI-MS was performed using an LTQ-Orbitrap XL spectrometer (Thermo Fisher Scientific, Boston, MA, USA). Acchrom S6000 high-performance liquid chromatograph (Acchrom Tech Technology Co., Ltd., Beijing, China) was used for analysis with an ELSD-UM 5800 Plus (Unimicro Technologies Co., Ltd., Shanghai, China). Column chromatography (CC) analysis was performed using silica gel (200–300 mesh and 300–400 mesh, Qingdao Ocean Chemical Factory, Qingdao, China) and C₁₈ reverse silica gel packing (50 μm, YMC Co., Ltd., Tokyo, Japan). Analytically pure solvents (petroleum ether, ethyl acetate, ethanol, methanol, n-BuOH, and dichloromethane) (Beijing Chemical Factory, Beijing, China) were used for extraction and CC separation. Chromatographically pure methanol (Thermo Fisher Scientific, Waltham, MA, USA) was used for high-performance liquid phase analysis. Deuterated solvents (Deuterated methanol, deuterated pyridine, deuterated chloroform, and deuterated dimethyl sulfoxide) (Aladdin Biochemical Technology Co., Ltd., Shanghai, China) were used for nuclear magnetic resonance spectroscopy.

Wu J., Li L., Liu C., Li C., Cui Y., Ding W., Zhang J, & Shi L. (2023). Two New Compounds from Allii Macrostemonis Bulbus and Their In Vitro Antioxidant Activities. Molecules, 28(17), 6176.

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Pharmacological Modulators of Ion Channels

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Tetrodotoxin (TTX), indomethacin, atropine, cimetidine, pyrilamine, and ketotifen were purchased from Sigma-Aldrich (Sigma-Aldrich, St. Louis, MO, USA). Petroleum ether, chloroform, ethyl acetate, and n-butanol were purchased from Beijing Chemical Works (Beijing, China). The Krebs solution had the following composition (mM): NaCl, 117; KCl, 4.5; CaCl₂, 2.5; MgCl₂, 1.2; NaHCO₃, 24.8; KH₂PO₄, 1.2; glucose, 11.1; pH 7.4.

Han W., Xu J.D., Wei F.X., Zheng Y.D., Ma J.Z., Xu X.D., Wei Z.G., Wang W, & Zhang Y.C. (2015). Prokinetic Activity of Prunus persica (L.) Batsch Flowers Extract and Its Possible Mechanism of Action in Rats. BioMed Research International, 2015, 569853.

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Comprehensive Analytical Procedure for Assessing Phytochemical Composition

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Acetonitrile 99.9%, n-hexane 95% and m ethanol 99.9% were purchased from Fisher Scientific (Shanghai, China). The fatty acids methyl ester (FAME) reference standard mixture 37 (standard 47885-U) was purchased from Sigma (St. Louis, MO, USA). Sugars standards [glucose, sorbitol and d(+)-trehalose and myo-inositol], phenolic standards (gallic, 4-hydroxybenzoic, 3,4-dihydroxybenzaldehyde, homogentisic, p-coumaric and o-coumaric acids) were purchased from Sigma (St. Louis, MO, USA) or Sigma-Aldrich Chem (shanghai, China). 1,1-Diphenyl-2-picryl-hydrazyl (DPPH), BHT, 3-(2-pyridyl)-5,6-bis (4-phenyl-sulfonic acid)-1,2,4-triazine (ferrozine), rutin and ascorbic acid were purchased from Sigma Chemical Co. BSTFA + TMCS (99:1) was purchased from Supelco. Folin–Ciocalteu’s phenol reagent was purchased from Beijing Solarbio reagent company (Beijing, China). Ferrous chloride, sodium salicylate, ferrous sulphate, hydrogen peroxide, potassium ferricyanide, trichloroacetic acid, disodium phosphate, sodium dihydrogen phosphate, ferric chloride, sodium carbonate, sodium hydroxide, aluminium nitrate, sodium nitrite, petroleum ether (60–90 °C), ethanol and m ethanol were all purchased from Beijing chemical plant (Beijing, China). All chemicals were of analytical grade. Water was treated in a Milli-Q water purification system (TGI Pure Water Systems, USA).

Yan X., Wang Y., Sang X, & Fan L. (2017). Nutritional value, chemical composition and antioxidant activity of three Tuber species from China. AMB Express, 7, 136.

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Synthesis and Characterization of Fluorinated Monomers

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Hexafluoroisopropyl methacrylate(HFIPMA) was obtained from Apollo Scientific Ltd. 2,2,3,3-Tetrafluoropropyl methacrylate(TFPMA), 2,2,2-trifluoroethyl methacrylate(TFEMA), 1H,1H,2H,2H-Nonafluorohexyl methacrylate(NFHMA) and 2,2,3,3,3-pentafluoropropyl methacrylate(PFPMA) were gotfrom TCI and these five kinds of monomers were used without any further purification. Lauroyl peroxide (LRPO, 97%) was recrystallized in Methanol before using. Benzotrifluoride (99%) was purchased from J&K Scientific (Beijing, China). PtTFPP was commercially available from Frontier Scientific Inc. Methanol (99.8%) (Logan, UT, USA) and petroleum ether were obtained from Beijing Chemical Works (Beijing, China). Scheme 1 presents their chemical structures.

Shao Y., Chen L., Liao Q., Jiang H., Liu Z., Jin X, & Gao L. (2020). Characterization of Response Frequency for the Pressure-Sensitive Paint Based on Fluorine-Containing Polymer Matrixes Using Oscillating Sound Wave Technique. Sensors (Basel, Switzerland), 20(21), 6310.

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Extraction and Purification of Amygdalin

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MS- and high performance liquid chromatography (HPLC)-grade acetonitrile, methanol, and formic acid were purchased from Fisher Scientific (Fair Lawn, NJ, USA). Analytical grade ethanol, methanol, dichloromethane, petroleum ether, sulfuric acid, aluminum chloride, and other reagents were purchased from Beijing Chemical Plant (Beijing, China). Ultrapure water was obtained from Watsons (Guangzhou, China). Column chromatography (CC) silica gel, thin layer silica gel G, and thin layer silica gel GF₂₅₄ were purchased from Qingdao Marine Chemical Plant (Qingdao, China). AB-8 macroporous adsorption resin and Sephadex LH-20 gel were obtained from Solarbio (Beijing, China). SiliaSphere C₁₈ was obtained from Silicycle (Quebec City, Canada). Dimethyl sulfoxide-d₆ and methanol-d₄ were purchased from Cambridge Isotopes Laboratories (Andover, MA, USA). Amygdalin standard compound (purity >98%) was obtained from Chengdu Pusi Biotechnology Co., Ltd. (Chengdu, China).

Zhao Z., Liu Y., Zhang Y., Geng Z., Su R., Zhou L., Han C., Wang Z., Ma S, & Li W. (2022). Evaluation of the chemical proﬁle from four germplasms sources of Pruni Semen using UHPLC-LTQ-Orbitrap-MS and multivariate analyses. Journal of Pharmaceutical Analysis, 12(5), 733-742.

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Analytical Reagents for Scientific Research

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Petroleum ether, n-butanol, and glucose were purchased from the Beijing Chemical Factory (Beijing, China). Anhydrous ethanol and anhydrous methanol were purchased from Modern Oriental Science and Technology Development Company (Beijing, China). Sodium hydroxide, ferric trichloride, dipotassium hydrogen phosphate, potassium dihydrogen phosphate, and potassium chloride were purchased from Xilong Chemical Company (Shanghai, China). Sulfuric acid and trichloromethane were purchased from Kunshan Jincheng Reagent Company (Suzhou, China). Agar, yeast dipping powder, and tryptone (bio grade) were purchased from Lablead Reagent Company (Beijing, China). The CAT assay kit, SOD assay kit, and ROS assay kit were purchased from Biomed Reagent Company (Beijing, China). The RNA extraction kit, reverse transcription kit, and fluorescence quantitative polymerase chain reaction (PCR) kit were purchased from Novozymes Reagent Company (Beijing, China). Monosaccharide standards mannose, ribose, rhamnose, glucuronic acid, galacturonic acid, N-acetyl-glucosamine, glucose, N-acetyl-galactosamine, galactose, xylose, arabinose, and fucose were purchased from Macklin (Shanghai, China).

Zou Y., Qin X., Wang W., Meng Q, & Zhang Y. (2024). Anti-Aging Effect of Hemerocallis citrina Baroni Polysaccharide-Rich Extract on Caenorhabditis elegans. International Journal of Molecular Sciences, 25(1), 655.

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Isolation and Purification of Ranae Oviductus

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The petroleum ether, guanidine hydrochloride, and ammonium sulfate analytical grade were purchased from Beijing Chemical Factory (Beijing, China). The dithiothreitol (DTT) and trifluoroacetic acid (TFA) were purchased from Sigma-Aldrich (St. Louis, MO, USA). The HPLC-grade acetonitrile (MeCN) and HPLC-grade methanol were purchased from Fisher (Fisher Scientific, USA). The ultrapure water was obtained from a gradient water purification system (Water Purifier, Sichuan, China).
Ranae Oviductus, bullfrog oviduct, toad oviduct and frog oviduct were provided by Jilin Province Rana Industry Association which were collected from the Changbai Mountain area in the Jilin province of China. The specific location is shown on the map in Figure 1. Ten batches of Ranae Oviductus samples were collected from different regions from the main producing area of the Changbai Mountain range. The specific collection information is shown in Table 1.

Gan Y., Xiao Y., Wang S., Guo H., Liu M., Wang Z, & Wang Y. (2019). Protein-Based Fingerprint Analysis for the Identification of Ranae Oviductus Using RP-HPLC. Molecules, 24(9), 1687.

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