Aa mxpro3000

Manufactured by Thermo Fisher Scientific

The Aa MxPro3000 is a real-time PCR system designed for quantitative gene expression analysis. It features a compact design and user-friendly software interface. The system provides reliable and accurate results for a wide range of real-time PCR applications.

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Lab products found in correlation

Trizol, by Thermo Fisher Scientific (2 mentions) Transcriptor first strand cdna synthesis kit, by Thermo Fisher Scientific (2 mentions) Sybr greener qpcr mix, by Thermo Fisher Scientific (2 mentions) Cfx96 real time pcr detection system, by Bio-Rad (1 mentions) Lightcycler 480 detection system, by Roche (1 mentions)

2 protocols using aa mxpro3000

Quantitative RT-PCR Analysis of Gene Expression

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Total RNA was isolated using Trizol (Invitrogen) according to the manufacturer’s instructions. For quantitative reverse transcription PCR (qRT–PCR) analysis, 1 μg of total RNA was reverse transcribed to cDNA using the Transcriptor First-Strand cDNA Synthesis kit (Fermentas). qRT–PCR was performed in triplicate with aa MxPro3000 and SYBR GreenERqPCR mix (Invitrogen). For qPCR analysis CFX96 Real-Time PCR Detection System (BioRad, Hercules, CA, USA) was employed using RNA expression of target genes relative to β-actin was quantified by 2ΔΔCT method. The relative amount of specific mRNA was normalized to β-actin in each sample. Primer pairs are listed in Supplementary Table 1.

Pencik J., Philippe C., Schlederer M., Atas E., Pecoraro M., Grund-Gröschke S., Li W.(., Tracz A., Heidegger I., Lagger S., Trachtová K., Oberhuber M., Heitzer E., Aksoy O., Neubauer H.A., Wingelhofer B., Orlova A., Witzeneder N., Dillinger T., Redl E., Greiner G., D’Andrea D., Östman J.R., Tangermann S., Hermanova I., Schäfer G., Sternberg F., Pohl E.E., Sternberg C., Varady A., Horvath J., Stoiber D., Malcolm T.I., Turner S.D., Parkes E.E., Hantusch B., Egger G., Rose-John S., Poli V., Jain S., Armstrong C.W., Hoermann G., Goffin V., Aberger F., Moriggl R., Carracedo A., McKinney C., Kennedy R.D., Klocker H., Speicher M.R., Tang D.G., Moazzami A.A., Heery D.M., Hacker M, & Kenner L. (2023). STAT3/LKB1 controls metastatic prostate cancer by regulating mTORC1/CREB pathway. Molecular Cancer, 22, 133.

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Quantitative RNA Expression Analysis

Check if the same lab product or an alternative is used in the 5 most similar protocols

Total RNA was isolated using Trizol (Invitrogen) according to the manufacturer's instructions. For quantitative reverse transcription PCR (qRT–PCR) analysis, 1 μg of total RNA was reverse transcribed to cDNA using the Transcriptor First-Strand cDNA Synthesis kit (Fermentas). qRT–PCR was performed in triplicate with aa MxPro3000 and SYBR GreenERqPCR mix (Invitrogen). Real-time monitoring of PCR amplification was performed using the LightCycler 480 detection system (Roche). The relative amount of specific mRNA was normalized to β-actin in each sample. Primer pairs are listed in Supplementary Table 3.

Pencik J., Schlederer M., Gruber W., Unger C., Walker S.M., Chalaris A., Marié I.J., Hassler M.R., Javaheri T., Aksoy O., Blayney J.K., Prutsch N., Skucha A., Herac M., Krämer O.H., Mazal P., Grebien F., Egger G., Poli V., Mikulits W., Eferl R., Esterbauer H., Kennedy R., Fend F., Scharpf M., Braun M., Perner S., Levy D.E., Malcolm T., Turner S.D., Haitel A., Susani M., Moazzami A., Rose-John S., Aberger F., Merkel O., Moriggl R., Culig Z., Dolznig H, & Kenner L. (2015). STAT3 regulated ARF expression suppresses prostate cancer metastasis. Nature Communications, 6, 7736.

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