The specific siRNA for NFE2L2 (Silencer Select Pre-designed and Custom Designed siRNA, Ambion, Carlsbad, CA, USA) was kindly provided by Beate Niesler (Department of Human Molecular Genetics, University of Heidelberg). Cells were transfected with siRNA using Lipofectamine RNAiMAX reagent (Invitrogen, Carlsbad, CA, USA) to silence the expression of NFE2L2 in the cell line. RNA Isolation and mRNA quantification by qPCR was repeated as outlined earlier. mRNA expression levels of NFE2L2 and PGR in NFE2L2 downregulated cells were compared with NFE2L2 containing cells.
Silencer select pre designed and custom designed sirna
Manufactured by Thermo Fisher Scientific
Sourced in United States
Silencer Select Pre-designed and Custom Designed siRNA are short, double-stranded RNA molecules designed to target and silence specific genes. The core function of these siRNA products is to induce the degradation of target mRNA, thereby reducing the expression of the corresponding gene.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using silencer select pre designed and custom designed sirna
1
siRNA-Mediated Silencing of NFE2L2
2
siRNA Silencing of NFE2L2 Gene
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The specific siRNA for NFE2L2 (Silencer Select Pre-designed and Custom Designed siRNA, Ambion, Carlsbad, CA, USA) was kindly provided by Beate Niesler (Department of Human Molecular Genetics, University of Heidelberg). Cells were transfected with siRNA using Lipofectamine RNAiMAX reagent (Invitrogen, Carlsbad, CA, USA) to silence the expression of NFE2L2 in the cell lines. RNA isolation and mRNA quantification by qPCR was repeated as outlined above. mRNA expression levels of NFE2L2 and ESR1 in NFE2L2-downregulated cells were compared with NFE2L2-containing cells.
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