Anti nhp2l1

Manufactured by Abcam

Anti-NHP2L1 is an antibody that targets the NHP2L1 protein. NHP2L1 is a component of the small nuclear ribonucleoprotein complex, which is involved in pre-mRNA splicing. The antibody can be used for research purposes to detect and study the NHP2L1 protein.

Automatically generated - may contain errors

Lab products found in correlation

Anti actin, by Merck Group (1 mentions) Anti traf3, by Santa Cruz Biotechnology (1 mentions) Anti snrnp200, by Merck Group (1 mentions) Anti rela, by Santa Cruz Biotechnology (1 mentions) Anti ddx3x, by Fortis Life Sciences (1 mentions) Anti ddx60, by Abcam (1 mentions) Anti irf3, by Santa Cruz Biotechnology (1 mentions) Hrp conjugated secondary antibody, by Bio-Rad (1 mentions) Protease inhibitor cocktail, by Roche (1 mentions) Western lighting chemiluminescence reagent plus, by PerkinElmer (1 mentions) Horseradish peroxidase conjugated secondary antibody, by Agilent Technologies (1 mentions) Anti gapdh, by Santa Cruz Biotechnology (1 mentions) β tubulin, by Merck Group (1 mentions)

2 protocols using anti nhp2l1

Comprehensive Cell Lysis and Immunoblotting

Check if the same lab product or an alternative is used in the 5 most similar protocols

Cells were washed twice with ice-cold phosphate-buffered saline (PBS; Wisent), harvested and lysed in 10mM Tris-HCl, 100mM NaCl, 0.5% Triton X-100, pH7.6 with EDTA-free Protease Inhibitor Cocktail (Roche). Cell lysates were clarified by centrifugation at 13,000 g for 20 min at 4°C and subjected to sodium dodecyl sulfate-polyacrylamide gel (SDS-PAGE). Western blot analysis was performed using mouse anti-PHF5A (Abnova), anti-IRF3 (Santa Cruz), anti-TRAF3 (Santa Cruz), anti-RIG-I (Alexis Biochemicals), anti-ACTIN (Chemicon International), anti-TBK1 (Imgenex and Santa Cruz), anti-IKBKE (Santa Cruz), anti-TUBULIN (ICN), anti-GAPDH (RDI) and rabbit anti-SNRNP200 (Sigma-Aldrich), anti-SF3A1 (Santa Cruz), anti-RELA (Santa Cruz), anti-NHP2L1 (Abcam), anti-DDX3X (Bethyl), anti-DDX60 (Abcam), TRIF (Cell Signaling), anti-ISG56 (Novus Biologicals), anti-MDA5 (Alexis Biochemicals), anti-MAVS (Alexis Biochemicals), anti-IKBKE (eBioscience), STAT1 (ABCAM), STAT1 tyr701 (ABCAM), IFNAR1 (Santa Cruz) and anti-IRF3-P-ser386 (Abcam). HRP-conjugated secondary antibodies were from Bio-Rad. The chemiluminescence reaction was performed using the Western Lighting Chemiluminescence Reagent Plus (PerkinElmer).

Tremblay N., Baril M., Chatel-Chaix L., Es-Saad S., Park A.Y., Koenekoop R.K, & Lamarre D. (2016). Spliceosome SNRNP200 Promotes Viral RNA Sensing and IRF3 Activation of Antiviral Response. PLoS Pathogens, 12(7), e1005772.

+ Open protocol

+ Expand

Immunoblotting: Cellular Protein Quantification

Check if the same lab product or an alternative is used in the 5 most similar protocols

Lysates from the CEM or NALM6 cell lines were separated by electrophoresis on an SDS‐polyacrylamide gel. The proteins were then electroblotted onto a Hybond‐P PVDF membrane. Protein expression was analyzed using the primary antibodies anti‐GAPDH (Santa Cruz Biotechnology #sc‐20357) and anti‐NHP2L1 (Abcam #ab95958). Horseradish peroxidase–conjugated secondary antibodies were purchased from Agilent (Santa Clara). Phospho‐histone H3 (Ser10) clone 3H10 (Millipore #05‐806); Beta tubulin (Sigma‐Aldrich#C4585) Histone H3 (proteintech#17168‐1‐AP) were used. Sororin was provided by Dr. Jan‐Michael Peters from the Research Institute of Molecular Pathology, Vienna, Austria.

Diouf B., Wing C., Panetta J.C., Eddins D., Lin W., Yang W., Fan Y., Pei D., Cheng C., Delaney S.M., Zhang W., Bonten E.J., Crews K.R., Paugh S.W., Li L., Freeman BB 3.r.d., Autry R.J., Beard J.A., Ferguson D.C., Janke L.J., Ness K.K., Chen T., Zakharenko S.S., Jeha S., Pui C., Relling M.V., Eileen Dolan M, & Evans W.E. (2021). Identification of small molecules that mitigate vincristine‐induced neurotoxicity while sensitizing leukemia cells to vincristine. Clinical and Translational Science, 14(4), 1490-1504.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!