Log In Sign up
The largest database of trusted experimental protocols

Pampkα1 α2

Manufactured by Cell Signaling Technology
Visit Supplier

PAMPKα1/α2 is a lab equipment product offered by Cell Signaling Technology. It functions as a recombinant protein that can be used for in vitro studies.

Automatically generated - may contain errors

Lab products found in correlation

Goat anti rabbit ir 800 and goat anti mouse ir 680 antibodies, by LI COR (3 mentions) Western blot gels, by Thermo Fisher Scientific (3 mentions) Odyssey blocking buffer, by LI COR (3 mentions) Protein sample loading buffer, by LI COR (3 mentions) Primary antibodies for ampkα1 α2, by Cell Signaling Technology (2 mentions) Metformin, by Enzo Life Sciences (2 mentions) Ampkα1 α2, by Cell Signaling Technology (2 mentions) Nupage lds sample buffer 4, by Thermo Fisher Scientific (2 mentions) Nupage lds sample buffer 4x, by Thermo Fisher Scientific (1 mentions) Ampkα1, by Cell Signaling Technology (1 mentions) P raptor, by Cell Signaling Technology (1 mentions) P acc, by Cell Signaling Technology (1 mentions) Alexa fluor 680, by Thermo Fisher Scientific (1 mentions) α tubulin, by Santa Cruz Biotechnology (1 mentions) Ampkα1β1γ1, by Sino Biological (1 mentions) Primary antibodies for lkb1, by Santa Cruz Biotechnology (1 mentions) β actin, by Santa Cruz Biotechnology (1 mentions) Lc3b 1, by Cell Signaling Technology (1 mentions) Lc3b 2, by Cell Signaling Technology (1 mentions) Aicar, by LC Laboratories (1 mentions)

4 protocols using pampkα1 α2

1

Metformin Regulates AMPK and SIRT1

Check if the same lab product or an alternative is used in the 5 most similar protocols
Metformin was obtained from Enzo Life Sciences (Farmingdale, NY) Primary antibodies for AMPKα1/α2 and pAMPKα1/α2 were obtained from Cell Signaling Technology (Danvers, MA). Primary antibodies for SIRT1, GAPDH and PGC1-α were obtained from Abcam (Cambridge, MA). The Odyssey blocking buffer, LI-COR goat anti-rabbit IR-800 and goat anti-mouse IR-680 antibodies, and the 4X Protein Sample Loading Buffer were obtained from LI-COR Biotechnology (Lincoln, NE). The NuPAGE® LDS Sample Buffer (4X), and Western blot gels were purchased from Life Technologies (Grand Island, NY). All other chemicals were obtained from Sigma-Aldrich (St. Louis, MO).
Matsiukevich D., Piraino G., Lahni P., Hake P.W., Wolfe V., O’Connor M., James J, & Zingarelli B. (2017). METFORMIN AMELIORATES GENDER- AND AGE-DEPENDENT HEMODYNAMIC INSTABILITY AND MYOCARDIAL INJURY IN MURINE HEMORRHAGIC SHOCK. Biochimica et biophysica acta, 1863(10 Pt B), 2680-2691.
+ Open protocol
+ Expand
2

Quantitative Immunoblotting of AMPK Signaling

Check if the same lab product or an alternative is used in the 5 most similar protocols
Primary antibodies were used in the indicated dilutions: PARP (#9542, Cell Signaling) 1:1000, α-Tubulin (#B-5-1-2, Santa Cruz) 1:2500, pACC (#3661, Cell Signaling) 1:2000, tACC (#3676, Cell Signaling) 1:2000, pRAPTOR (#2083, Cell Signaling) 1:1000, tRAPTOR (#2280, Cell Signaling) 1:1000, pAMPKα1α2 (#2531, Cell Signaling) 1:1000, AMPKα1α2 (#2532, Cell Signaling) 1:1000, AMPKα2 (#AF2850, R&D systems) 1:1000, and AMPKα1 (#2795, Cell Signaling) 1:1000. Anti-mouse, and anti-rabbit secondary antibodies conjugated to Alexa Fluor 680 (Invitrogen, Carlsbad, CA) or IRDye800, and IRDye680LT were used at 1:5000-1:10,000 dilutions. Additional reagents included recombinant AMPKα1β1γ1 (#P47-10H, SignalChem) and AMPKα2β1γ1 (#P48-10H, SignalChem), SAMS peptide (S07-58, Cquential Solutions), and radioactive α-32P-ATP (64014, MPBIo).
Das B., Neilsen B.K., Fisher K.W., Gehring D., Hu Y., Volle D.J., Kim H.S., McCall J.L., Kelly D.L., MacMillan J.B., White M.A, & Lewis R.E. (2018). A Functional Signature Ontology (FUSION) screen detects an AMPK inhibitor with selective toxicity toward human colon tumor cells. Scientific Reports, 8, 3770.
+ Open protocol
+ Expand
3

Metformin Regulation of AMPK Signaling

Check if the same lab product or an alternative is used in the 5 most similar protocols
Metformin was obtained from Enzo Life Sciences (Farmingdale, NY). Primary antibodies for AMPKα1/α2 and pAMPKα1/α2 were obtained from Cell Signaling Technology (Danvers, MA). Primary antibodies for LKB1, pLKB1 and β-actin were obtained from Santa Cruz Biotechnology (Dallas, TX). Primary antibody for PGC1-α was obtained from Abcam (Cambridge, MA). The Odyssey blocking buffer, LI-COR goat anti-rabbit IR-800 and goat anti-mouse IR-680 antibodies, and the 4× Protein Sample Loading Buffer were obtained from LI-COR Biotechnology (Lincoln, NE). The Multiplex Luminex Array kit, the NuPAGE® LDS Sample Buffer (4×), and Western blot gels were purchased from Life Technologies (Grand Island, NY). All other chemicals were obtained from Sigma-Aldrich (St. Louis, MO).
Kim P., Piraino G., O’Connor M., Hake P.W., Wolfe V., Lahni P, & Zingarelli B. (2018). Metformin exerts beneficial effects in hemorrhagic shock in an AMPKα1-independent manner. Shock (Augusta, Ga.), 49(3), 277-287.
+ Open protocol
+ Expand
4

AICAR Signaling Pathway Analysis

Check if the same lab product or an alternative is used in the 5 most similar protocols
The AMP analog, AICAR, was obtained from LC Laboratories (Woburn, MA). Primary antibodies for AMPKα1/α2, pAMPKα1/α2, and LC3B-I and LC3B-II were obtained from Cell Signaling Technology (Danvers, MA). Primary antibodies for GAPDH and for PGC1-α were obtained from Abcam (Cambridge, MA). The Odyssey blocking buffer, LI-COR goat anti-rabbit IR-800 and goat anti-mouse IR-680 antibodies, and the 4× Protein Sample Loading Buffer were obtained from LI-COR Biotechnology (Lincoln, NE). The NuPAGE® LDS Sample Buffer (4×), and Western blot gels were purchased from Life Technologies (Grand Island, NY). All other chemicals were obtained from Sigma-Aldrich (St. Louis, MO).
Matsiukevich D., Piraino G., Klingbeil L.R., Hake P.W., Wolfe V., O’Connor M, & Zingarelli B. (2017). THE AMPK ACTIVATOR AICAR AMELIORATES AGE-DEPENDENT MYOCARDIAL INJURY IN MURINE HEMORRHAGIC SHOCK. Shock (Augusta, Ga.), 47(1), 70-78.
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!