P. putida 6157 (RIKEN, Saitama, Japan) was used for biofilm cultivation. All chemicals used for the LB media were purchased from Becton Dickinson (Sparks, MD, USA). The MALDI matrices, 2,5-dihydroxybenzoic acid (DHB), and α-cyano-4-hydroxycinnamic acid (CHCA), were purchased from Sigma-Aldrich (St. Louis, MO, USA). Methanol was purchased from WAKO Pure Chemical Industries (Osaka, Japan). Ultrapure water was obtained using a Genpure UV-TOC xCAD Plus (Thermo Fisher Scientific, Waltham, MA, USA).
Maldi matrices
Manufactured by Merck Group
Sourced in United States, Germany
MALDI matrices are compounds used in matrix-assisted laser desorption/ionization (MALDI) mass spectrometry. They facilitate the desorption and ionization of analyte molecules, enabling their detection and analysis by mass spectrometers. These matrices are designed to be compatible with a variety of analytes and sample types, supporting the broad applications of MALDI mass spectrometry in fields such as proteomics, lipidomics, and metabolomics.
Automatically generated - may contain errors
Lab products found in correlation
Methanol, by Fujifilm (1 mentions)
2 5 dihydroxybenzoic acid dhb, by Merck Group (1 mentions)
α cyano 4 hydroxycinnamic acid chca, by Merck Group (1 mentions)
Genpure uv toc xcad plus, by Thermo Fisher Scientific (1 mentions)
Microloader tip, by Eppendorf (1 mentions)
Chymotrypsin, by Merck Group (1 mentions)
Phosphatidylcholine, by Merck Group (1 mentions)
Trypsin, by Merck Group (1 mentions)
Pepsin, by Merck Group (1 mentions)
4 protocols using maldi matrices
1
Biofilm Cultivation of P. putida
2
MALDI Matrices Sourced Globally
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The different MALDI matrices were purchased from Sigma-Aldrich (Darmstadt, Germany).
3
Metabolite Analysis using MALDI-MS
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
All solvents were purchased from Thermo Fisher Scientific (Waltham, USA). The indium thin oxide (ITO)-coated glass slides were obtained from Bruker Daltonik (Bremen, Germany). The MALDI matrices as well as pure metabolite compounds were purchased from Sigma-Aldrich (Taufkirchen, Germany). The 10 μl tips and microloader tips were purchased from Eppendorf (Hamburg, Germany).
4
Characterizing Dairy Powders and Enzymatic Digestion
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Samples of CN, SMP and WPI powders were provided by the Fonterra Dairy Co. via the Riddett Institute (New Zealand). Porcine pancreatic enzymes (pepsin >2,500 U/mg, trypsin >10,000 BAEE U/mg, chymotrypsin > 40 U/mg and elastase >4 U/mg), solvents and chemicals, including phosphatidylcholine, bile salts/acids (taurocholate/glicodeoxycholic acid) and MALDI matrices were purchased from Sigma (St. Louis, MO, USA). Carboxypeptidase A was from Roche (Mannheim, Germany).
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!