Normal human CFs were obtained from ScienCell Research Laboratories (San Diego, CA, USA). TGF-β1 was obtained from R&D Systems, Inc., (Minneapolis, MN, USA). Antibodies directed against α-smooth muscle actin (α-SMA), collagen type Iα (COLA)-1, COLA3, cyclin-dependent kinase 1 (CDK1), cyclin B, phospho-smad2/3 (p-smad2/3), phospho-p38 mitogen-activated protein kinase (p-p38 MAPK), phospho-extracellular regulated protein kinases (p-ERK), and GAPDH were purchased from Bioss Antibodies (Beijing, China).
P p38 mapk
Manufactured by Bioss Antibodies
Sourced in China
P-p38 MAPK is a lab equipment product that detects the phosphorylated form of the p38 mitogen-activated protein kinase (MAPK). It is used to measure the activation of the p38 MAPK signaling pathway.
Automatically generated - may contain errors
Lab products found in correlation
P38 mapk, by Bioss Antibodies (2 mentions)
Gapdh, by Bioss Antibodies (1 mentions)
P erk, by Bioss Antibodies (1 mentions)
Tgf β1, by R&D Systems (1 mentions)
Flag tag (flag), by Beyotime (1 mentions)
Pgc 1α, by ZenBio (1 mentions)
P smad1 5 8, by Affinity Biosciences (1 mentions)
Ha tag, by Beyotime (1 mentions)
P acc, by Sangon (1 mentions)
Goat anti rabbit igg hrp secondary antibody, by CWBIO (1 mentions)
P ikkα β, by Bioss Antibodies (1 mentions)
β actin, by Bioss Antibodies (1 mentions)
Pparγ, by Affinity Biosciences (1 mentions)
C ebpα, by Bioss Antibodies (1 mentions)
P erk1 2, by Bioss Antibodies (1 mentions)
Camkiiδ, by Abcam (1 mentions)
Gapdh, by Wuhan Servicebio Technology (1 mentions)
Tgf β1, by Abcam (1 mentions)
3 protocols using p p38 mapk
1
Investigating Fibroblast Activation Pathways
2
Immunoblotting for Adipogenesis Regulators
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Antibodies from Affinity Biosciences: p-Smad1/5/8 (1:1000, #AF8313), Smad1/5/8 (1:1000, #AF0614), PPARγ (1:1000, #bs-4590R), UCP1 (1:1000, #72298). Antibodies from Beyotime: HA tag (1:1000, #AH158), Flag tag (1:1000, #AF519). Antibodies from Sangon Biotech: p-ACC (1:1000, #D155180), ACC (1:1000, #D155300). Antibodies from Abcam: BMP8A (1:1000, #154373). Antibodies from ZenBio: p-AMPK (1:1000, #R26252), AMPK (1:1000, #380431), PGC-1α (1:1000, #381615). Antibodies from CWBIO: goat anti-Rabbit IgG HRP secondary antibody (1:4000, #CW0103S), goat anti-Mouse IgG HRP Conjugated (1:4000, # CW0102). Antibodies from Bioss: p38 MAPK (1:1000, #bs-0637R), p-p38 MAPK (1:1000, #bs-2210R), β-actin (1:2000, #bs-0061R), p-JNK (1:1000, #bs-1640R), JNK (1:1000, #bs-2592R), C/EBPα (1:1000, #AF6333), p-Smad2/3 (1:1000, #AF3367), Smad2/3 (1:1000, #AF6367), p-ERK1/2 (1:1000, #AF1015), ERK1/2 (1:1000, #AF0155), p-p65 (1:1000, #AF2006), p65 (1:1000, #AF5006), p-IKKα/β (1:1000, #AF3013), IKKα/β (1:1000, # AF6014).
3
Western Blot Analysis of Atrial Proteins
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Tissues from the left atrium (LA) were used for Western blotting, which was performed based on our previous study (20 (link)). Membranes were blotted with antibodies against NOX2 (1:1,000; Abcam), NOX4 (1:1,000; Abcam), CaMKIIδ (1:1,000, Abcam), p-CaMKIIδ (1:1,000, Bioss), p38MAPK (1:1,000, Bioss), p-p38MAPK (1:1,000, Bioss), Cav1.2 (1:1,000, Abcam), Kv4.2 (1:1,000, Abcam), Kv4.3 (1:1,000, Abcam), TGF-β1 (1:1,000, Abcam), and collagen I (1:1,000, Bioss). GAPDH (1:1,000, Servicebio) was used as a reference protein.
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