Rabbit anti cxcl12
Rabbit anti-CXCL12 is a primary antibody that recognizes the CXCL12 protein. CXCL12, also known as stromal cell-derived factor 1 (SDF-1), is a chemokine that plays a role in cellular processes such as cell migration and adhesion. This antibody can be used for the detection and quantification of CXCL12 in various applications.
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6 protocols using rabbit anti cxcl12
Western Blot Analysis of CXCL12/CXCR4 Signaling
Femur Histological Analysis Protocol
Other sections, after rinsing with PBS and incubation with 0,3% H2O2 were incubated with blocking buffer (0.3% Triton X-100 and 1% BSA in PBS) containing 10% normal serum for 30-60 min at RT in a humidified chamber. Then, sections were incubated 1 h a RT with the following primary antibodies diluted 1:80 in blocking buffer: rabbit anti-Osterix (Santa Cruz Biotechnology, DBA, Milano, Italy); rabbit anti-CXCL12 (Abcam; Prodotti Gianni, Milano, Italy); mouse anti-nestin (Abcam; Prodotti Gianni, Milano, Italy). After washing in PBS, sections were incubated for 30 min at RT with a biotinylated goat anti-mouse IgG (Vector Laboratories, DBA Milano, Italy) or with a biotinylated goat anti-rabbit IgG (Bethyl Laboratories, Aurogene s.r.l., Roma, Italy) both diluted 1:200 in blocking buffer. Control experiments were performed by omitting the primary antibody. Slides were imaged using a Leica DM 2500 microscope.
Protein Expression Analysis of Spinal Cord
Western Blot Analysis of CXCL12 and CXCR4
Immunofluorescence Analysis of CD34+ Cells
Western Blot Analysis of Bone Cells
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