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Anti mouse b220 bv510

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Anti-mouse B220-BV510 is a flow cytometry reagent that binds to the B220 (CD45R) surface marker on mouse B cells. It is used to identify and enumerate B cells in flow cytometry applications.

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Lab products found in correlation

Lsm 710, by Zeiss (4 mentions) Tissue tek optimum cutting temperature compound, by Sakura Finetek (2 mentions) B220 alexa fluor 647 ra3 6b2, by BioLegend (2 mentions) Anti mouse cd45.2 alexa fluor 488 clone 104, by BioLegend (2 mentions) Cas block, by Thermo Fisher Scientific (2 mentions)

2 protocols using anti mouse b220 bv510

1

Multicolor Immunostaining of Frozen Tissues

Check if the same lab product or an alternative is used in the 5 most similar protocols
Fresh spleens or LNs were snap-frozen in Tissue-Tek optimum cutting temperature (O.C.T.) compound (Sakura Finetek). Cryosections of 10 μm were cut, fixed in 4% of paraformaldehyde for 10 min followed by another 10 min incubation in pre-chilled acetone at −20 °C, and then washed three times with PBS for immunostaining. Following incubation in blocking buffer (CAS-Block, Invitrogen), the samples were incubated with fluorescence-labeled antibodies overnight at 4 °C. The primary antibodies used are anti-mouse CD8-Alexa Fluor 594 (53-6.7), anti-mouse B220-BV510 or B220-Alexa Fluor 647 (RA3-6B2 for both fluorochromes), and anti-mouse CD45.2-Alexa Fluor 488 (clone 104, all from BioLegend). Confocal images of cryosections were acquired using a Zeiss LSM710 confocal fluorescence microscopy and were processed with Imaris software (Bitplane).
Shan Q., Zeng Z., Xing S., Li F., Hartwig S.M., Gullicksrud J.A., Kurup S.P., Van Braeckel-Budimir N., Su Y., Martin M.D., Varga S.M., Taniuchi I., Harty J.T., Peng W., Badovinac V.P, & Xue H.H. (2017). Runx3 guards cytotoxic CD8+ effector T cells against deviation towards TFH cell lineage. Nature immunology, 18(8), 931-939.
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2

Multicolor Immunostaining of Frozen Tissues

Check if the same lab product or an alternative is used in the 5 most similar protocols
Fresh spleens or LNs were snap-frozen in Tissue-Tek optimum cutting temperature (O.C.T.) compound (Sakura Finetek). Cryosections of 10 μm were cut, fixed in 4% of paraformaldehyde for 10 min followed by another 10 min incubation in pre-chilled acetone at −20 °C, and then washed three times with PBS for immunostaining. Following incubation in blocking buffer (CAS-Block, Invitrogen), the samples were incubated with fluorescence-labeled antibodies overnight at 4 °C. The primary antibodies used are anti-mouse CD8-Alexa Fluor 594 (53-6.7), anti-mouse B220-BV510 or B220-Alexa Fluor 647 (RA3-6B2 for both fluorochromes), and anti-mouse CD45.2-Alexa Fluor 488 (clone 104, all from BioLegend). Confocal images of cryosections were acquired using a Zeiss LSM710 confocal fluorescence microscopy and were processed with Imaris software (Bitplane).
Shan Q., Zeng Z., Xing S., Li F., Hartwig S.M., Gullicksrud J.A., Kurup S.P., Van Braeckel-Budimir N., Su Y., Martin M.D., Varga S.M., Taniuchi I., Harty J.T., Peng W., Badovinac V.P, & Xue H.H. (2017). Runx3 guards cytotoxic CD8+ effector T cells against deviation towards TFH cell lineage. Nature immunology, 18(8), 931-939.
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