Norepinephrine ne hydrochloride

All chemicals used were of analytical grade. Reagents were stored in hard dark glass bottles with glass stoppers to avoid leaching of fluorescent contaminants: acidified N-butanol (0.85 mL of concentrated HCl was added to 1 L of N-butanol), N-heptane, hydrochloric acid (0.1 N and 10 N), sodium hydroxide (5 N), acetic acid (0.2 N and 5 N), iodine (0.1 N; 1.27 g of iodine was dissolved in 100 mL of absolute ethanol), EDTA (0.12 M; 0.1-M EDTA was dissolved in 1-N sodium acetate and the pH was adjusted to 7.0 with 5-N sodium hydroxide), NaHCO₃ (0.033 M), and alkaline sulphite [25% (W/V), 0.5 g of anhydrous sodium sulphite was dissolved in 2 mL distilled water]. This solution was mixed well with 18 mL of 1 N NaOH just before use. O-phthalaldehyde (OPT; 4 mg and 10 mg) was dissolved in 10-N hydrochloric acid. Dopamine (DA) hydrochloride, norepinephrine (NE) hydrochloride, serotonin (5-HT), and hydrogen oxalate (obtained from Sigma-Aldrich, St. Louis, USA) were used in the experiments.