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Mouse ige elisa kit

Manufactured by BioLegend
Sourced in United States

The Mouse IgE ELISA kit is a quantitative sandwich immunoassay designed for the measurement of mouse immunoglobulin E (IgE) levels in serum, plasma, and cell culture supernatants.

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3 protocols using mouse ige elisa kit

1

Cytokine and Chemokine Profiling in Lung Tissue

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Th1 (IL-2, IFN-γ)/Th2 (IL-4, IL-5, IL-13) cytokine and TNF-α levels in supernatants of lung tissue lysates were determined using CBA Flex Set Kits (BD Biosciences) with a FACScan flow cytometer equipped with CellQuest Pro™ (BD Biosciences) for data acquisition and FlowJo Software (Tree Star, Inc.) for analysis. Eotaxin-1 and eotaxin-2 in the BALF and IL-33 in lung lysates was measured using ELISA kits (R&D Systems). IgE level in the BALF was measured using a mouse IgE ELISA kit (BioLegend). All kits were used as per the manufacturer’s recommendation.
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2

Mouse IgE ELISA Quantification

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IgE concentrations were measured using a mouse IgE ELISA kit (BioLegend, Inc., San Diego, CA) according to the manufacturer’s protocol.
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3

Intestinal Immunoglobulin Quantification

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The levels of IgA, IgE, IgM, and IgG in the intestinal mucosa were analyzed using a mouse IgA ELISA kit (eBioscience, Santa Clara, CA, USA), mouse IgE ELISA kit (Biolegend), mouse IgM ELISA kit (Biolegend), and mouse Total IgG ELISA kit (eBioscience) according to the manufacturers’ instructions. The plates were read at 450 nm.
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