Mouse ccr2 apc conjugated antibody

DMAB-anabaseine dihydrochloride and PHA568487 were from Tocris Biosciences (Minneapolis, MN, USA); (−)-Nicotine hemisulfate salt and LPS E. coli 0111:B4 were from Sigma (St Louis, MO, USA). Anti-mouse Ly6C APC, anti-mouse CD11b PE, anti-mouse Gr1 FITC and PE, anti-mouse CD4 FITC and PE, anti-mouse CD16/CD32 antibodies and IgG isotype controls were from eBioscience (San Diego, CA, USA). For immunofluorescence, anti-mouse CD11b and Gr1 antibodies were from BD Biosciences (San Jose, CA, USA). Phospho-Akt1 (Ser473) (D7F10) XP rabbit mAb (Akt1 Specific) and PathScan Phospho-Akt1 (Ser473) Sandwich ELISA Kit were from Cell Signaling (Danvers, MA, USA); AChRα7 antibody (H-302) was from Santa Cruz Biotechnology (Santa Cruz, CA, USA). Mouse CCR2 APC-conjugated antibody and mouse CXCL2 and TNF-α ELISA Kit were from R&D Systems (Minneapolis, MN, USA). Anti-Choline acetyltransferase antibody was from Abcam (Cambridge, MA, USA). E. coli K1 (serotype) strain, isolated from patients with biliary infection, was kindly provided by Dr Thomas Martin (University of Washington, USA) [69 (link)].

The following anti-mouse antibodies, including Lineage cocktail (17A2; RB6-8C5, RA3-6B2; Ter-119; M1/70); CD117 (cKit, 2B8), Ly-6A/E (Sca-1, D7), CD3 (145-2C11), CD19 (6D5), B220 (RA3-6B2), Ly-6C (HK1.4), Ly-6G (1A8), CD45.1 (A20), CD24 (M1/69), and CX3CR1 (SA011F11) were purchased from Biolegend. Ki-67-FITC staining set was purchased from BD Biosciences. Mouse CCR2 APC-conjugated antibody was purchased from R&D Systems. Anti-myeloperoxidase (MPO) antibody (2D4) FITC was purchased from Abcam. Intracellular fixation and permeabilization solution kit was purchased from BD. Violet cell proliferation kit were purchased from Thermo Fisher Scientific. Cyclophosphamide monohydrate (CTX) was purchased from Tokyo Chemical Industry. 5-azacytidine was purchased from Selleckchem. Anti-mouse TNFa (XT3.11) mAb was purchased from Bio X Cell. Mouse IL6 neutralization mAb was purchased from Biolegend.