Brain tissue specimens were cut in the transverse plane and prepared as paraffin-embedded sections. For immunofluorescence examination, the sections were incubated overnight at 4 °C with mouse anti-cleaved-caspase 3 (1:100; Affinity Biosciences, OH, USA) and rabbit anti-caspase 12 (1:200; Proteintech North America, IL, USA) primary antibodies. The sections were washed three times and incubated with the following biotinylated secondary antibodies: FITC-conjugated AffiniPure goat anti-rabbit IgG (1:200, BOSTER, Wuhan, China) and CY3-conjugated AffiniPure goat anti-mouse IgG (1:200, BOSTER, Wuhan, China). The nuclei were stained with DAPI (BOSTER, Wuhan, China). Fluorescence images were acquired using an Olympus BX53 fluorescence microscope (Olympus Corporation, Tokyo, Japan). Finally, antigen–antibody reactions were developed using diaminobenzidine. The Image-Pro Plus software (Media Cybernetics, Silver Spring, MD, USA) was used for semiquantitative analysis.
Fitc conjugated affinipure goat anti rabbit igg
Manufactured by Boster Bio
Sourced in China
FITC-conjugated AffiniPure goat anti-rabbit IgG is a secondary antibody conjugated with fluorescein isothiocyanate (FITC). It is designed to detect and visualize rabbit primary antibodies in various immunoassays.
Automatically generated - may contain errors
Lab products found in correlation
4 6 diamidino 2 phenylindole (dapi), by Boster Bio (1 mentions)
Cy3 conjugated affinipure goat anti mouse igg, by Boster Bio (1 mentions)
Bx53 fluorescence microscope, by Olympus (1 mentions)
Image pro plus software, by Media Cybernetics (1 mentions)
Dapi staining solution, by Boster Bio (1 mentions)
Sp8 sted 3x, by Leica camera (1 mentions)
Anti m13 antibody, by Abcam (1 mentions)
2 protocols using fitc conjugated affinipure goat anti rabbit igg
1
Caspase-3 and Caspase-12 Immunofluorescence in Brain Tissue
2
Phage-Cell Binding Assay Protocol
Check if the same lab product or an alternative is used in the 5 most similar protocols
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SKOV3, Hela, AN3CA, MCF7, MHCC97-H and HEK293 cells (4 × 104/well) were cultured on coverslips overnight and then fixed with 4% paraformaldehyde for 30 min at room temperature. Followed by washing with PBS three times and blocking with 1% BSA for 30 min at 37 °C, 1 × 1011 pfu representative clone of positive phage and IRP were added and incubated with the cells at 37 °C for 2 h. Cells were washed with PBS and incubated with anti-M13 antibody (Abcam, USA) at a dilution of 1:200 at 4 °C overnight. Subsequently, cells were washed with PBST and FITC Conjugated AffiniPure Goat Anti-rabbit IgG (working dilution of 1:100, Boster Biological Technology Co., Ltd., China) were added and incubated at 37 °C for 2 h. After washing three times with PBS, DAPI-Staining-solution (Boster Biological Technology Co., Ltd., China) was used to stain the nucleus. The cells were finally observed using an inverted microscope (SP8 STED 3X, Leica, Germany). IRP and PBS were used as control groups. The relative intensity of green fluorescence signal was calculated using Image J.
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