Annexin 5 pe propidium iodide apoptosis detection kit

Manufactured by BD

Sourced in China

The Annexin V-PE/propidium iodide (PI) apoptosis detection kit is a laboratory instrument used to detect and quantify apoptosis, a type of programmed cell death, in cell samples. The kit utilizes Annexin V, a protein that binds to phosphatidylserine, and propidium iodide, a fluorescent dye that binds to DNA, to identify different stages of apoptosis in cells.

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Lab products found in correlation

Tunel kit, by Roche (1 mentions)

Close spelling variants of this product

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2 protocols using annexin 5 pe propidium iodide apoptosis detection kit

Apoptosis Assay by Flow Cytometry

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The cells were harvested in 0.25% trypsin and washed twice with PBS. The degree of apoptosis in cells was assessed using the Annexin V-PE/propidium iodide (PI) apoptosis detection kit (BD, China) by flow cytometric analysis. The experiments were performed in triplicate. The results represent the means ± SD. CytExpert software was used to analyze the ratio of apoptotic cells.

Zhang M., Peng Y., Yang Z., Zhang H., Xu C., Liu L., Zhao Q., Wu J., Wang H, & Liu J. (2022). DAB2IP down-regulates HSP90AA1 to inhibit the malignant biological behaviors of colorectal cancer. BMC Cancer, 22, 561.

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Apoptosis Assessment in Cells and Tissues

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The cells were harvested in 0.25% trypsin and washed twice with PBS. The degree of apoptosis in cells was assessed using the Annexin V-PE/propidium iodide (PI) apoptosis detection kit (BD, Shanghai, China) by flow cytometric analysis. The degree of apoptosis in tissue was assessed with the TUNEL kit (Roche) according to the manufacturer’s instructions. In addition, western blot analyses for c-PARP and analysis of caspase 3 activity by Colorimetric Caspase 3 Assay Kit (Merck) after various treatments were performed.

Xiao X., Wang W., Li Y., Yang D., Li X., Shen C., Liu Y., Ke X., Guo S, & Guo Z. (2018). HSP90AA1-mediated autophagy promotes drug resistance in osteosarcoma. Journal of Experimental & Clinical Cancer Research : CR, 37, 201.

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