Viscotek 270

To analyse the M_W of all polymers, gel permeation chromatography (GPC) was performed. The GPC system consisted of a pump and injection system (Viscotek GPCmax, Malvern Instruments Ltd, UK) combined with a refractive index (RI) detector (Viscotek VE3580, Malvern Instruments Ltd, UK), multiple angle light scattering detector (Viscotek SEC-MALS 20, laser wavelength 660 nm, Malvern Instruments Ltd, UK) and a viscosity detector (Viscotek 270, Malvern Instruments Ltd, UK). For the measurements, an aqueous eluent 0.1 M NaNO₃ with 0.02% NaN3 for conservation at a flow rate of 0.7 ml min⁻¹ with 100 μl injection volume was used. For separation, two polymethyl methacrylate columns (A6000, Malvern Instruments Ltd, UK) and a precolumn all kept at 35 °C were applied. Each sample was injected 5 times (n = 5). The decrease of M_W was calculated as follows: with M_W (ALG) as the molecular weight of ALG and M_W (sample) as the molecular weight of the sample.

Molecular mass of p-PGF was determined using gel-permeation chromatography (GPC) on a chromatograph Malvern equipped with double detector Viscotek 270 (Malvern Instruments INC, Bristol, United Kingdom). According to the results of the experiment, the molecular weight of the polymer was Mw—2630 Da. Polystyrene was used as a standard, and dioxane was used as a solvent. Data were found out with the help of a light scattering (LS) detector (Malvern Instruments INC, Bristol, United Kingdom). The experiment was carried out by universal calibration (UC), the dn/dc index of the sample was 0.0839, the column type was T6000M for organic solvents, the current rate was 1.0 mL/min, and the temperature was 40 °C.