Agents used in this study including PFD (300 mg/kg/day, Kangdini, Beijing), Captopril (CAP, 20 mg/kg/day, MedChemExpress), antibodies against α‐smooth muscle actin (α‐SMA, ABclonal, 1:1000), Vimentin (ABclonal, 1:1000), TGF‐β1 (ABclonal, 1:1000), phosphorylated‐TGFβ receptor 1‐S165 (p‐TGFβR1S165, Affinity Biosciences, 1:1000), TGFβ receptor 1 (TGFβR1, ABclonal, 1:1000), phosphorylated‐Smad3‐S423/425 (p‐Smad3S423/S425, ABclonal, 1:1000), total Smad3 (ABclonal, 1:1000), Smad7 (ABclonal, 1:1000), glyceraldehyde‐3‐phosphate dehydrogenase (GAPDH, ABclonal, 1:10,000) were all commercially purchased.
Tgfβr1
Manufactured by ABclonal
TGFβR1 is a receptor protein that binds to the TGF-beta signaling molecule. It plays a key role in the TGF-beta signaling pathway, which is involved in various cellular processes such as cell growth, differentiation, and immune regulation.
Automatically generated - may contain errors
Lab products found in correlation
α sma, by ABclonal (1 mentions)
Gapdh, by ABclonal (1 mentions)
Goat anti rabbit igg h l, by Beyotime (1 mentions)
P smad2, by Abcam (1 mentions)
P smad3, by Abcam (1 mentions)
P jnk1 2, by Cell Signaling Technology (1 mentions)
P erk1 2, by ABclonal (1 mentions)
Erk1 2, by ABclonal (1 mentions)
Collagen 3, by Abcam (1 mentions)
Smad2 3, by Cell Signaling Technology (1 mentions)
α sma, by Abcam (1 mentions)
Tgf β1, by ABclonal (1 mentions)
Cdh11, by Abcam (1 mentions)
Erk1 2 inhibitor, by Selleck Chemicals (1 mentions)
Angiotensin 2 a9525, by Merck Group (1 mentions)
Ly3200882, by Selleck Chemicals (1 mentions)
U0126, by Selleck Chemicals (1 mentions)
Smad4, by Cell Signaling Technology (1 mentions)
Sp600125, by Selleck Chemicals (1 mentions)
Sb203580, by Selleck Chemicals (1 mentions)
2 protocols using tgfβr1
1
Investigating TGF-β Signaling Pathway
2
Antibody Profiling of Signaling Pathways
Check if the same lab product or an alternative is used in the 5 most similar protocols
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The following antibodies were purchased: CDH11 (Abcam, 4442), vimentin (Abcam, ab8978), collagen-III (Abcam, ab34710), α-SMA (Abcam, ab5694), JNK (Cell Signaling, #9252), pJNK1/2 (Cell Signaling, #9251), ERK1/2 (Abclonal, A10613), pERK1/2 (Abclonal, AP0472), P38 (Abclonal, A11340), pP38 (Abclonal, AP0297), Smad2/3 (Cell Signaling), p-Smad2 (Abcam), p-Smad3 (Abcam), Smad4 (Cell Signaling), TGF-β1 (Abclonal), TGF-βR1 (Abclonal), GAPDH (Beyotime, AG019) and goat anti-rabbit IgG H&L (Beyotime, A0423). Angiotensin-II (A9525) was purchased from Sigma-Aldrich and hIgG1 (110-HG) and hCDH11-Fc (1790-CA) were from R&D Systems. LY3200882 (TGF-β-Smad pathway inhibitor), U0126 (ERK1/2 inhibitor), SP600125 (JNK inhibitor) and SB203580 (P38-MAPK inhibitor) were purchased from Selleck Chemicals (USA).
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