To detect survivin gene expression, ovarian serous carcinoma sections were antigen-retrieved and incubated with blocking buffer (5% normal goat serum, 3% bovine serum albumin, and 0.1% Triton-X100 in PBS) for 1 h. The slides were incubated overnight with primary antibodies to survivin (1:200 dilution, Cell Signaling, Danvers, MA). After rinsing three times for 5 min with PBST, Alexa 488- or 594- conjugated goat anti-rabbit (Invitrogen, Carlsbad, CA) antibodies were added for 1 h at room temperature. Cell nuclei were counterstained with DAPI (Vector Laboratories, Inc.; Burlingame, CA). Images were captured on a Zeiss LSM700 laser scanning confocal microscope.
Alexa 488 or 594 conjugated goat anti rabbit
Manufactured by Thermo Fisher Scientific
Alexa Fluor 488- or 594-conjugated goat anti-rabbit antibodies are secondary antibodies that bind to rabbit primary antibodies. These conjugated antibodies are designed for use in immunofluorescence and other fluorescence-based detection techniques.
Automatically generated - may contain errors
Lab products found in correlation
3 protocols using alexa 488 or 594 conjugated goat anti rabbit
1
Survivin Expression in Ovarian Serous Carcinoma
2
Detecting Survivin and EMT Markers in Ovarian Cancer
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To detect survivin and EMT markers in human ovarian cancer specimens or cell lines, ovarian tumor sections were antigen-retrieved by heating sections in sodium citrate buffer (10 mM sodium citrate, 0.05% Tween, pH 6.0) for 30 mins, while ovarian cancer cells were fixed in 4% paraformaldehyde for 30 mins. Sections or cells were incubated with blocking buffer (5% normal goat serum, 3% bovine serum albumin, and 0.1% Triton-X100 in PBS) for 1 h, and then incubated overnight with primary antibodies to survivin, PCNA, cytokeratin 7 and vimentin (1:200 dilution, Cell Signaling, Danvers, MA). After rinsing three times for 5 min with PBST, samples were incubated for 1 h at room temperature with Alexa 488- or 594-conjugated goat anti-rabbit (Invitrogen, Carlsbad, CA) antibodies. Cell nuclei were counterstained with DAPI (Vector Laboratories, Inc.; Burlingame, CA). Images were captured on a fluorescent microscope (Nikon, San Diego, CA).
3
Detecting EMT and MTF1 in Ovarian Cancer
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To detect MTF1 and EMT marker expression in human ovarian cancer specimens or cell lines, ovarian serous carcinoma sections were antigen-retrieved by heating sections in sodium citrate buffer(10mM sodium citrate,0.05%Tween, pH6.0) for 30 mins, whereas ovarian cancer cells were fixed in 4% paraformaldehyde for 30 mins. Sections or cells were incubated with blocking buffer (5% normal goat serum, 3% bovine serum albumin, and 0.1% Triton-X100 in PBS) for 1 h and then incubated overnight with primary antibodies to MTF1, PCNA, cytokeratin 7, and β-catenin (1:200 dilution, Cell Signaling, Danvers, MA). After rinsing three times for 5 min with PBST, Alexa 488- or 594-conjugated goat anti-rabbit (Invitrogen, Carlsbad, CA) antibodies were incubated for 1 h at room temperature. Cell nuclei were counterstained with DAPI (Vector Laboratories, Inc.; Burlingame, CA). Images were captured under a fluorescent microscope (Zeiss model LSM700, San Diego, CA). Imunofluoresent intensity was measured using Image J software on tumor and adjacent non-tumor area from six different sections of human ovarian serous carcinoma and mean intensity was used to compare the significance using Student's t-test.
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