Anti gsdmd ab219800

Manufactured by Abcam

Sourced in United States

Anti-GSDMD (ab219800) is a primary antibody that recognizes the GSDMD (Gasdermin D) protein. GSDMD is a key mediator of pyroptosis, a form of inflammatory programmed cell death. This antibody can be used in various applications to detect and study the GSDMD protein.

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Lab products found in correlation

Strataclean resin, by Agilent Technologies (1 mentions) Atp tlrl atpl, by InvivoGen (1 mentions) Aicar s1802, by Selleck Chemicals (1 mentions) Compound c p5499, by Merck Group (1 mentions) Anti ha h6908, by Merck Group (1 mentions) Pt172 ampk, by Cell Signaling Technology (1 mentions) Anti il18 a1115, by ABclonal (1 mentions) Cd8 percp cy5.5 clone 53 6 7, by BioLegend (1 mentions) Cd4 apc clone rm4 5, by BioLegend (1 mentions) Dulbecco s modified eagle s medium dmem, by Thermo Fisher Scientific (1 mentions) Anti nf κb p65 8242, by Cell Signaling Technology (1 mentions) Fetal bovine serum (fbs), by Thermo Fisher Scientific (1 mentions) Lipopolysaccharide lps escherichia coli 055 b5, by Merck Group (1 mentions) Nf κb p50, by Cell Signaling Technology (1 mentions)

Close spelling variants of this product

GSDMD (79 citations) Ab219800 (73 citations) Anti-GSDMD (23 citations)

2 protocols using anti gsdmd ab219800

AMPK Signaling Pathway Modulation

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LPS O111:B4 (L2630), Doxycycline (D3447), 2-Deoxy-D-glucose (D8375) and compound C (P5499) were obtained from Sigma-Aldrich. Subcellular protein fractionation kit (78840) was obtained from Thermo Fisher. Metformin (S1950) and AICAR (S1802) were obtained from Selleck Chemicals. ATP (tlrl-atpl) was obtained from InvivoGen. StrataClean resin (400714) and chemical competent cells (200315) were obtained from Agilent.
For immunoblot, AMPKα1(#2795), AMPKα2 (#2757), AMPKα1/2 (#5831) and pT172-AMPK (#2535) were purchased from Cell Signaling Technology. Anti-Flag (F1804), anti-Flag (F7425) and anti-HA (H6908) were obtained from Sigma-Aldrich. Anti-GSDMD (ab219800) and anti-Caspase-1 (ab108362) were obtained from Abcam. Anti-β-Actin (sc-47778), anti-GST (sc-138) and anti-Na/K-ATPase α1 (sc-21712) were purchased from Santa Cruz Biotechnology. Anti-IL18 (A1115) was obtained from Abclonal Technology. phospho-GSDMD S46 was generated by Abclonal Technology.
For FCAS analysis, CD45-APC-Cy7(clone 30-F11), CD3-FITC (clone 145-2C11), CD8-PerCP-Cy5.5 (clone 53-6.7) and CD4-APC (clone RM4-5) were obtained from BioLegend.

Chu X., Xiao X., Wang G., Uosef A., Lou X., Arnold P., Wang Y., Kong G., Wen M., Minze L.J, & Li X.C. (2023). Gasdermin D-mediated pyroptosis is regulated by AMPK-mediated phosphorylation in tumor cells. Cell Death & Disease, 14(7), 469.

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LPS-Induced Inflammation Modulation

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Mangiferin (MF), purity>99.84%, was purchased from the Shanghai Winherb Medical Co. (Shanghai, China). Lipopolysaccharide (LPS) (Escherichia coli 055:B5) was obtained from Sigma-Aldrich (St. Louis, MO, USA). Anti-β-actin (ab6276), anti-NLRP3 (ab263899), anti-ASC (ab151700) and anti-GSDMD (ab219800) were obtained from Abcam (Cambridge, MA, USA). Anti-NF-κB p65 (#8242) and NF-κB p50 (#3035) were provided by Cell Signalling Technology (Danvers, MA, USA). Dulbecco’s modified Eagle’s medium (DMEM) and fetal bovine serum (FBS) were obtained from Invitrogen-Gibco (Grand Island, NY). The secondary antibody was provided by LI-COR Biosciences (Lincoln, United States). All chemicals used in this study were of analytical grade.

Li N., Xiong R., He R., Liu B., Wang B, & Geng Q. (2021). Mangiferin Mitigates Lipopolysaccharide-Induced Lung Injury by Inhibiting NLRP3 Inflammasome Activation. Journal of Inflammation Research, 14, 2289-2300.

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