Lvdv 2 cone and plate viscometer

Example 30

The viscosity of each polymer drug conjugated sample was measured on a Brookfield LVDV-II Cone and Plate viscometer fitted with a temperature controlled jacketed plate. The polymer sample (0.5 mL of a 10, 20, 30 and 40% w/w solution in water) was placed on the center of the plate, which was attached to the main drive of the instrument. The cone (CPE-40) was rotated at different rates (rpm) and the viscosity (mPas) was recorded each time at 25° C. The table below shows a comparison of viscosity readings for each sample tested. The results show that POZ conjugates of the present disclosure have low viscosity that allow for ease of administration through a narrow bore needle.

Example 30

The viscosity of each polymer drug conjugated sample was measured on a Brookfield LVDV-II Cone and Plate viscometer fitted with a temperature controlled jacketed plate. The polymer sample (0.5 mL of a 10, 20, 30 and 40% w/w solution in water) was placed on the center of the plate, which was attached to the main drive of the instrument. The cone (CPE-40) was rotated at different rates (rpm) and the viscosity (mPas) was recorded each time at 25° C. The table below shows a comparison of viscosity readings for each sample tested. The results show that POZ conjugates of the present disclosure have low viscosity that allow for ease of administration through a narrow bore needle.

Example 1

10 ml of a selected anti-sclerostin antibody (75.7 mg/ml) was dialyzed against 2 liters of 10 mM Na(OAc) and 9% sucrose at 4° C. for 2 hours. A selected anti-sclerostin antibody (75.7 mg/ml) was concentrated to approximately 160 mg/ml and diluted with water to approximately 140 mg/ml and 120 mg/ml. Absorbance of the diluted samples were determined to be 120, 142 and 157 mg/ml, respectively

10 μl 1.0M Ca(OAc)₂ was added to 1 ml of the 120 mg/ml, 140 mg/ml and 160 mg/ml samples. Absolute viscosity, pH and osmolarity of the samples were determined (See Table 2). Absolute viscosity of the samples (500 μl) was measured using Brookfield LV-DVII cone and plate viscometer with a CPE-40 spindle with matching sample cup temperature regulated by a circulating water bath at constant 25° C.

Results indicated that 10 mM Ca(OAc)₂ spiked into a liquid composition of the selected antibody reduced viscosity by about half. This experiment is performed for each of antibodies Ab-4, Ab-5, Ab-13, Ab-14, Ab-19, Ab-20 and Ab-23.