Anti cd4 epr19514

Manufactured by Abcam

Sourced in United States, United Kingdom

Anti-CD4 (EPR19514) is a recombinant rabbit monoclonal antibody that recognizes the CD4 antigen. CD4 is a cell surface glycoprotein that is involved in T-cell activation and differentiation. This antibody can be used in various applications, such as flow cytometry and immunohistochemistry, to detect and analyze CD4-positive cells.

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Lab products found in correlation

Opal 7 color automation ihc kit, by Akoya Biosciences (1 mentions) Mantra quantitative pathology workstation, by PerkinElmer (1 mentions) Inform cell analysis software, by PerkinElmer (1 mentions) Bond rxm automated stainer, by Leica (1 mentions) Anti pan cytokeratin ck rabbit poly, by Bioss Antibodies (1 mentions) Anti cd8α epr20305, by Abcam (1 mentions) Ra3 6b2, by Abcam (1 mentions)

Close spelling variants of this product

Anti-CD4 (59 citations) EPR19514 (10 citations)

2 protocols using anti cd4 epr19514

Histological Analysis of NIR-PIT Treatment

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Histological changes after NIR-PIT were evaluated. Tumors were extracted 7 days after each NIR-PIT. Extracted tumors were then fixed with 10% formalin, embedded in paraffin, thinly sliced, stained with Hematoxylin and Eosin (H&E) staining.
The multiplex IHC is performed with Opal 7-Color Automation IHC Kit (Akoya Bioscience, Hopkinton, MA, USA) and BOND Rxm automated stainer (Leica Biosystems; Wetzlar, Germany) using the following antibodies; anti-pan-cytokeratin (CK) (rabbit poly; Bioss, Woburn, MA, USA), anti-CD4 (EPR19514; Abcam, Cambridge, UK), anti-CD8α (EPR20305; Abcam) and anti-Foxp3 (1054C; Novus Biologicals, Centennial, CO).
The slides were imaged in the Mantra Quantitative Pathology Workstation (PerkinElmer, Waltham, MA) and analyzed with inForm Cell Analysis software (PerkinElmer).

Okada R., Maruoka Y., Furusawa A., Inagaki F., Nagaya T., Fujimura D., Choyke P.L, & Kobayashi H. (2019). The Effect of Antibody Fragments on CD25 Targeted Regulatory T Cell Near Infrared Photoimmunotherapy (NIR-PIT). Bioconjugate chemistry, 30(10), 2624-2633.

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Immunohistochemistry of Lymphoid Tissues

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Formalin-fixed, paraffin-embedded samples including the spleen, thymus, and lymph nodes, of 8-week-old homozygous null and wild-type mice were used for immunohistochemical staining. Anti-B220 (RA3-6B2; Abcam, Cambridge, UK) and anti-CD4 (EPR19514; Abcam) antibodies were used for B cell and T cell immunostaining, respectively.

Lee J.H., Park J.H., Nam T.W., Seo S.M., Kim J.Y., Lee H.K., Han J.H., Park S.Y., Choi Y.K, & Lee H.W. (2018). Differences between immunodeficient mice generated by classical gene targeting and CRISPR/Cas9-mediated gene knockout. Transgenic Research, 27(3), 241-251.

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