Fluorescence emission spectra between 330 and 480 nm (excitation
at 315 nm) were recorded at room temperature with a Jobin Yvon Horiba
Fluorolog 3 fluorescence spectrometer (Longjumeau, France) for two
samples of 10 μM Aβ40 peptide in 20 mM MES
buffer, pH 7.3. One sample contained 100 μM uranyl acetate to
investigate the possible effects of uranyl ions on dityrosine formation.
The control sample contained 100 μM of the chelator EDTA to
remove any free metal ions. All measurements were conducted in triplicate
using a quartz cuvette with a 4 mm path length and containing a 0.7
mL liquid sample. Spectra were recorded after 0 and 24 h of incubation,
during which the samples were kept at room temperature without agitation
or other treatment.
at 315 nm) were recorded at room temperature with a Jobin Yvon Horiba
Fluorolog 3 fluorescence spectrometer (Longjumeau, France) for two
samples of 10 μM Aβ40 peptide in 20 mM MES
buffer, pH 7.3. One sample contained 100 μM uranyl acetate to
investigate the possible effects of uranyl ions on dityrosine formation.
The control sample contained 100 μM of the chelator EDTA to
remove any free metal ions. All measurements were conducted in triplicate
using a quartz cuvette with a 4 mm path length and containing a 0.7
mL liquid sample. Spectra were recorded after 0 and 24 h of incubation,
during which the samples were kept at room temperature without agitation
or other treatment.