Vaginal smears were assessed from weeks 6–8 of treatment. All female rats were vaginally lavaged daily with 10 µL of saline solution (NaCl 0.9%), and cells were immediately viewed under a microscope before behavioral assessment. Images were observed with an inverted fluorescence microscope (TE 3000, Nikon Instruments Inc, New York, NY). Vaginal smears were stained using an adaptation of the Papanicolaou (PAP) stain developed by Dr. George N. Papanicolaou.33 The original PAP staining used a regressive method, where tissue was overstained followed by the removal of excess stain.
Te 3000
Manufactured by Nikon
Sourced in United States
The TE 3000 is a high-performance laboratory microscope system designed for a variety of scientific applications. It features a sturdy and ergonomic design, providing a stable platform for precise observation and analysis. The TE 3000 offers a range of advanced optical capabilities, including high-quality lenses and illumination systems, to ensure reliable and accurate results.
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Lab products found in correlation
4 protocols using te 3000
1
Vaginal Cytology Assessment in Rats
2
Uptake of Cy3-Tagged Extracellular Vesicles by HMVECs
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Uptake of ssEVs by HMVECs was examined by treating the HMVECs with Cy3‐tagged ssEVs. The ssEVs were tagged with Cy3 by loading Cy3 siRNA using Exo‐Fect siRNA/miRNA Transfection Kit (Cat. #: EXFT200A‐1, System Biosciences) according to the manufacturer's protocol. Then HMVECs were treated with Cy3‐tagged ssEVs (108 particles/mL) for 48 hours. Uptake of Cy3‐tagged ssEVs was evaluated using a fluorescent microscope (Nikon TE3000).
3
Long-term Imaging of Neurons under Gradient
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For long-term imaging of the neurons under a gradient, the device was placed in an environmental chamber (Pathology Devices, Baltimore, MD, USA), which was fitted to the stage of an inverted epifluorescence microscope (Nikon TE 3000). The heat input was adjusted using a controller to maintain the temperature of the cell culture surface and the media at 37 °C during the course of the experiment. Pre-mixed 5% CO2 gas was bubbled through water and fed into the chamber. Holes were drilled on the side of the chamber to fit the tubing and connect it to the fluidic ports on the device. The microscope was fitted with Nikon’s Perfect Focus objectives that eliminate focus drift for long-term imaging. Phase contrast and fluorescence images were obtained with a 12-bit cooled CCD camera (ORCA ER, Hamamatsu, Japan). The stage-movement, the automated acquisition of the phase contrast and the fluorescence images for different chamber positions were controlled using the Nikon Elements software.
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3D-Printed Devices Imaging and Analysis
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