Nkp44 pe cy7

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NKP44-PE-CY7 is a fluorochrome-conjugated antibody product used for flow cytometry analysis. The antibody specifically binds to the NKp44 receptor, which is expressed on the surface of natural killer cells.

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2 protocols using nkp44 pe cy7

Characterization of Primary Human NK Cells

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NK cell markers expression was verified using mouse anti-human antibodies to CD45-APC-CY7, CD25-FITC, CD16-PE, CD3-PE-CY7, CD56-PAC BLUE and CD122-PE, were from BD Biosciences. Anti-human antibodies to NKG2D-APC, MHC-1 HLA-A2-APC, NKP30-PE, NKP44-PE-CY7, NKP46-FITC, Granzyme B-FITC, Perforin1-PE, Interferon-γ-APC, TNF-α1- APC-CY7, were from BioLegend, DAPI from Invitrogen, mouse anti-cMyc: sureLight APC was from Columbia Biosciences. Cells were sorted at MGH Flow Cytometry Core facility using a BD 5 laser SORP FACS Vantage SE Diva system (BD Biosciences). FACS data and ∑Median statistics were analyzed using FlowJo Software (Tree Star, Inc.). Human primary NK (hNK) cells were extracted from peripheral blood of healthy donors using the Rosettesep™ human enrichment kit (StemCell technologies).

Jounaidi Y., Cotten J.F., Miller K.W, & Forman S.A. (2017). Tethering IL2 to its receptor IL2Rβ enhances anti-tumor activity and expansion of natural killer NK92 cells. Cancer research, 77(21), 5938-5951.

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Profiling NK Cell Subsets and Receptors

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In order to analyze NK cell subsets and their receptor expression profile, PBMCs were labeled with anti-CD3-BV785, -CD16-BV570, -CD38-BV510, -CD56-BV711, -CD57-FITC, -NKG2A-PE, -NKG2D-PE/Cy7, -NKp30-BV421, -NKp44-PE/Cy7 and -NKp46-AlexaFluor700 mAbs (all from Biolegend, San Jose, CA, USA) for 20 minutes at room temperature in the dark. The data were collected and analyzed with NovoCyte flow cytometer with NovoExpress operating system software (Agilent Technologies, USA).

Gelmez M.Y., Oktelik F.B., Tahrali I., Yilmaz V., Kucuksezer U.C., Akdeniz N., Cetin E.A., Kose M., Cinar C., Oguz F.S., Besisik S., Koksalan K., Ozdemir O., Senkal N., Gul A., Tuzun E, & Deniz G. (2022). Immune modulation as a consequence of SARS-CoV-2 infection. Frontiers in Immunology, 13, 954391.

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