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4 protocols using d methionine

1

Arginine and Methionine Acquisition Protocol

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DL-arginine 98% was purchased from Alfa Aesar (Schiltigheim, France), L-arginine 98%, fumaric acid 97% and D-Methionine 98% were purchased from Acros Organics (Geel, Belgium), 13C6-L-arginine.HCl 99% was purchased from Cambridge Isotope Laboratories (Andover, MA, USA). Methanol and ethanol were of HPLC grade and purchased from VWR (Fontenay-sous-Bois, France). Analytical grade formic acid was purchased from Sigma Aldrich (Saint-Quentin Fallavier, France). Water was ultra-purified (18.2 MΩ) with an Elga system from Veolia water solution & technologies (Saint-Maurice, France).
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2

Preparation of D-Methionine Saline Solution

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In a sterile hood environment, 99+% pharmaceutical-grade D-methionine (Acros Organics) was dissolved into 0.9% sterile saline at 30 mg/ml. The solution was then stored in the refrigerator until use.
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3

Synthesis of Metal Nanoparticles

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HAuCl4 ∙ 3H2O (99.99% (metals basis)), L-cysteine hydrochloride (98%), D-cysteine hydrochloride (98%), L-glutathione (98%+), D-methionine (99%), L-methionine (98 + %), L-cystine dihydrochloride (98%), D-cystine (98%), Cu(NO3)2 trihydrate (98%), Fe(NO3)3 nonahydrate (98%), Co(NO3)2 hexahydrate (99%), Ni(NO3)2 hexahydrate (98%), and KOH (99 + %) were purchased from Alfa Aesar. AgNO3 (>99.9%), HCl (37%), and H2SO4 (75%) were purchased from Roth. Hexadecyl-trimethylammonium bromide (CTAB, >99%), ascorbic acid (AA, >99%), and acetic acid (80%), were purchased from Acros Organics. Hexadecyltrimetryammonium chloride (CTAC, >98.0%), 1-decanol (>98%), L-penicillamine (99%), D-penicillamine (99%), Cd(NO)3 tetrahydrate (99%), Pb(NO3)2 (99%), and ammonium hydroxide (28%–30%) were purchased from Sigma-Aldrich. Milli Q water was used in all experiments. All reagents were used as received without further purification.
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4

Amino Acid Supplementation for Cell Culture

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Dulbecco’s modified Eagle’s medium (DMEM), phenol-free DMEM, penicillin and streptomycin, trypsin, and fetal bovine serum (FBS) were purchased from Pan Biotech (Aidenbach, Germany). High glucose DMEM without methionine and cysteine were purchased from Sigma Aldrich (Istanbul, Turkey). Cell culture supplements such as normocin were purchased from Invivogen (Toulouse – France) and hypoxanthine (HAT) supplements from LGC Standards (Istanbul, Turkey). Transfection reagent Polyjet was purchased from Signagen (Maryland, USA). D-amino acids D-alanine, D-arginine, D-phenylalanine, D-methionine, and D-tryptophan were purchased from Alfa Aesar (Landau, Germany). D-serine, D-valine, were purchased from Sigma Aldrich (Istanbul, Turkey). D-cysteine was purchased from ChemCruz (Heidelberg, Germany). P-Azido-L-phenylalanine (pAzF) was purchased from Bachem (Bubendorf, Swiss). L-Alanine and all chemicals to prepare imaging media and buffer solutions were purchased from neoFroxx (Einhausen, Germany).
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