Sanco μct 40

Manufactured by Scanco

Sourced in Switzerland

The Sanco μCT 40 is a compact, high-resolution micro-computed tomography (micro-CT) system designed for non-destructive 3D imaging and analysis of small samples. It utilizes advanced X-ray technology to capture detailed, high-quality images of the internal structures of various materials, including biological, geological, and industrial samples.

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Lab products found in correlation

Phosphotungstic acid hydrate, by Merck Group (2 mentions)

2 protocols using sanco μct 40

Soft Tissue Imaging via PTA Staining

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Phosphotungstic acid (PTA) staining was performed to increase soft tissue conspicuity for specimen computed tomography (CT) imaging. Lungs were inflated and fixed with 10% formalin at 20 cm H₂O pressure for seven days. Samples were initially washed 3X in 70% EtOH in 50 ml non-reactive tubes prior to staining. Each lung was then immersed in 0.3% (w/v) Phosphotungstic acid hydrate (Sigma-Aldrich P4006) in 70% EtOH for seven days on an oscillating table. They were subsequently air dried prior to imaging.
Specimen CT scanning of the dried lungs was performed on a Sanco μCT 40 (ScanCo Medical AG, Switzerland. Imaging was performed at 70kVP at 114 μA current and 200 ms integration time. Images were reconstructed using a conebeam algorithm at 16 μm voxel size in a DICOM file format. Images were viewed with ImageJ.

Dinnon KH I.I.I., Leist S.R., Okuda K., Dang H., Fritch E.J., Gully K.L., De la Cruz G., Evangelista M.D., Asakura T., Gilmore R.C., Hawkins P., Nakano S., West A., Schäfer A., Gralinski L.E., Everman J.L., Sajuthi S.P., Zweigart M.R., Dong S., McBride J., Cooley M.R., Hines J.B., Love M.K., Groshong S.D., VanSchoiack A., Phelan S.J., Liang Y., Hether T., Leon M., Zumwalt R.E., Barton L.M., Duval E.J., Mukhopadhyay S., Stroberg E., Borczuk A., Thorne L.B., Sakthivel M.K., Lee Y.Z., Hagood J.S., Mock J.R., Seibold M.A., O’Neal W.K., Montgomery S.A., Boucher R.C, & Baric R.S. (2022). A model of persistent post SARS-CoV-2 induced lung disease for target identification and testing of therapeutic strategies. bioRxiv.

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PTA-Enhanced Lung Specimen Imaging

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Phosphotungstic acid (PTA) staining was performed to increase soft tissue conspicuity for specimen computed tomography (CT) imaging. Lungs were inflated and fixed with 10% formalin at 20 cm H₂O pressure for seven days. Samples were initially washed 3X in 70% EtOH in 50 mL non-reactive tubes prior to staining. Each lung was then immersed in 0.3% (w/v) Phosphotungstic acid hydrate (Sigma-Aldrich P4006) in 70% EtOH for seven days on an oscillating table. They were subsequently air dried prior to imaging.
Specimen CT scanning of the dried lungs was performed on a Sanco μCT 40 (ScanCo Medical AG). Imaging was performed at 70kVP at 114 μA current and 200 ms integration time. Images were reconstructed using a conebeam algorithm at 16 μm voxel size in a DICOM file format. Images were viewed with ImageJ and analyzed by blinded radiologists (M.K.S. and Y.Z.L).

Dinnon KH I.I.I., Leist S.R., Okuda K., Dang H., Fritch E.J., Gully K.L., De la Cruz G., Evangelista M.D., Asakura T., Gilmore R.C., Hawkins P., Nakano S., West A., Schäfer A., Gralinski L.E., Everman J.L., Sajuthi S.P., Zweigart M.R., Dong S., McBride J., Cooley M.R., Hines J.B., Love M.K., Groshong S.D., VanSchoiack A., Phelan S.J., Liang Y., Hether T., Leon M., Zumwalt R.E., Barton L.M., Duval E.J., Mukhopadhyay S., Stroberg E., Borczuk A., Thorne L.B., Sakthivel M.K., Lee Y.Z., Hagood J.S., Mock J.R., Seibold M.A., O’Neal W.K., Montgomery S.A., Boucher R.C, & Baric R.S. (2022). SARS-CoV-2 infection produces chronic pulmonary epithelial and immune cell dysfunction with fibrosis in mice. Science Translational Medicine.

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