The largest database of trusted experimental protocols

Goat antirabbit igg h l af 647 preadsorbed secondary antibody

Manufactured by Abcam
Sourced in United Kingdom

Goat anti-rabbit IgG H&L (AF-647) preadsorbed secondary antibody is a highly specific and cross-adsorbed secondary antibody that recognizes the heavy and light chains of rabbit immunoglobulin G (IgG). It is conjugated with Alexa Fluor 647, a fluorescent dye with excitation/emission maxima of 650/665 nm, allowing for detection and visualization in various applications.

Automatically generated - may contain errors

2 protocols using goat antirabbit igg h l af 647 preadsorbed secondary antibody

1

Histological Analysis of Cardiac Tissue

Check if the same lab product or an alternative is used in the 5 most similar protocols
Hearts were sectioned in the coronal plane to expose the four chambers, the aorta and the pulmonary trunk. The defected present in the LV apex represents the location of the catheter that was inserted to decompress the left ventricle during Langendorff type B perfusion (LV vent). Following each experiment, the heart was transferred to 10% neutral buffered formalin and paraffin embedded within 48 h. Using a microtome, 5 μm sections were prepared and stained with H&E, Masson’s trichrome (muscle: red; nucleus: blue; green/blue: collagen), and labeled with rabbit monoclonal recombinant anti-CD31 primary antibody (Abcam, Cambridge, UK) and fluorescent tagged with goat antirabbit IgG H&L (AF-647) preadsorbed secondary antibody (Abcam). The sections were also labeled with Isolectin Griffonia simplicifolia AF-488 conjugate (Thermo Fisher, Waltham, MA) which binds d-galactosyl residues of galactose α−1,3 galactose (Gal α−1,3 Gal). Nuclei were labeled with 4′,6-diamidina-2-phenylindole (DAPI).
+ Open protocol
+ Expand
2

Histological Analysis of Cardiac Tissue

Check if the same lab product or an alternative is used in the 5 most similar protocols
Hearts were sectioned in the coronal plane to expose the four chambers, the aorta and the pulmonary trunk. The defected present in the LV apex represents the location of the catheter that was inserted to decompress the left ventricle during Langendorff type B perfusion (LV vent). Following each experiment, the heart was transferred to 10% neutral buffered formalin and paraffin embedded within 48 h. Using a microtome, 5 μm sections were prepared and stained with H&E, Masson’s trichrome (muscle: red; nucleus: blue; green/blue: collagen), and labeled with rabbit monoclonal recombinant anti-CD31 primary antibody (Abcam, Cambridge, UK) and fluorescent tagged with goat antirabbit IgG H&L (AF-647) preadsorbed secondary antibody (Abcam). The sections were also labeled with Isolectin Griffonia simplicifolia AF-488 conjugate (Thermo Fisher, Waltham, MA) which binds d-galactosyl residues of galactose α−1,3 galactose (Gal α−1,3 Gal). Nuclei were labeled with 4′,6-diamidina-2-phenylindole (DAPI).
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!