Rabbit ab against c jun n terminal kinases jnk

Antibodies (Abs) and ELISA kits for detecting IL-8 and AMTB hydrochloride (AMTB) were purchased from R&D Systems (Minneapolis, MN, USA). Rabbit Ab against c-Jun N-terminal kinases (JNK) was obtained from Cell Signaling (Beverly, MA, USA). Mouse Ab against phospho-JNK was purchased from BD Biosciences (San Jose, CA, USA). Mouse Abs against extracellular signal-regulated kinase (ERK), phospho-ERK, histone 1 (H-1), and rabbit Ab against p65 and nuclear factor-like 2 (Nrf2) were obtained from Santa Cruz Biotechnology (Santa Cruz, CA, USA). Mouse Ab against α-tubulin, mouse Ab against FLAG, ethylene glycol tetraacetic acid (EGTA), N-acetyl-cysteine (NAC), apocynin, and L-menthol (purity > 99%, FCC) were purchased from Sigma-Aldrich (St. Louis, MO, USA). Rabbit Ab against TRPM8 and 4-Hydroxynonenal (4-HNE) were obtained from Abcam (Cambridge, MA, USA). The Screen Quest™ Fluo-8 Medium Removal Calcium Assay Kit was purchased from AAT Bioquest (Sunnyvale, CA, USA). The membrane-permeable probe hydroethidine (HE) was purchased from Molecular Probes (Eugene, OR, USA). Scramble and TRPM8 siRNAs were obtained from GE Dharmacon (Lafayette, CO, USA). INTERFERin siRNA transfection reagent was purchased from Polyplus (New York, NY, USA).

Antibodies (Abs) and ELISA kits for the detection of IL-8 and MIP-2 were purchased from R&D Systems (Minneapolis, MN, USA). Rabbit Ab against c-Jun N-terminal kinases (JNK) was obtained from Cell Signaling (Beverly, MA, USA). Mouse Ab against phospho-JNK was purchased from BD (San Jose, CA, USA). Abs against extracellular signal-regulated kinase (ERK), phospho-ERK, and p65 were obtained from Santa Cruz Biotechnology (Santa Cruz, CA, USA). Mouse Abs against α-tubulin, HC030031, ethylene glycol tetraacetic acid (EGTA), N-acetyl-cysteine, and apocynin were purchased from Sigma-Aldrich (St. Louis, MO, USA). Mouse Ab against TRPA1 was obtained from Abcam (Cambridge, MA, USA) or from Calbiochem (San Diego, CA, USA). Mouse Ab against histone H1 was purchased from Millipore (Bedford, MA, USA). Mouse Ab against 4-hydroxynonenal (4-HNE) was obtained from Abcam (Cambridge, MA, USA). The Screen Quest Fluo-8 Medium Removal Calcium Assay Kit was purchased from AAT Bioquest (Sunnyvale, CA, USA). The EnzyChrom NADP⁺/NADPH assay kit was obtained from BioAssay Systems (Hayward, CA, USA). The membrane-permeable probes hydroethidine (HE) and 2′,7′-dichlorofluorescin diacetate (DCFH-DA) were purchased from Molecular Probes (Eugene, OR, USA). Scramble and TRPA1 siRNAs were obtained from Ambion (Austin, TX, USA). INTERFERin siRNA transfection reagent was purchased from Polyplus (New York, NY, USA).