Mv4 11

Manufactured by Lonza

Sourced in United States, Belgium

The MV4-11 is a laboratory instrument designed for cell culture and cell biology applications. It functions as an automated cell counter and viability analyzer. The MV4-11 utilizes advanced imaging technology to provide accurate cell counts and viability measurements.

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Lab products found in correlation

Trypan blue exclusion assay, by Lonza (1 mentions) Opti gold, by GenDEPOT (1 mentions) Antibiotic antimycotic, by Lonza (1 mentions) Mv4 11, by Leibniz Institute DSMZ (1 mentions) Fetal bovine serum (fbs), by Biowest (1 mentions) Rpmi 1640 medium, by Lonza (1 mentions) Pcmv neo bam p53 r248w, by Addgene (1 mentions) Aml 193, by American Type Culture Collection (1 mentions) Amaxa nucleofector, by Lonza (1 mentions) Mv4 11, by American Type Culture Collection (1 mentions) Thp 1 tib 202, by American Type Culture Collection (1 mentions) Penicillin streptomycin solution, by Merck Group (1 mentions) Fetal calf serum (fcs), by Merck Group (1 mentions) 1640 medium, by Lonza (1 mentions)

3 protocols using mv4 11

Cultivation and Characterization of Acute Myeloid Leukemia Cell Lines

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Acute Myeloid Leukemia cell lines U937 and MV4-11 were purchased from the Deutsche Sammlung von Mikroorganismen und Zellkulturen (DSMZ, Braunschweig, Germany). U937 cells were cultured in RPMI 1640 medium (Lonza, Walkersville, MD, USA) supplemented with 10% (v/v) fetal bovine serum (FBS), Biowest, Riverside, MO, USA) and 1% antibiotic–antimycotic (Lonza, Walkersville, MD, USA). MV4-11 cells were cultures in RPMI 1640 medium (Lonza, Walkersville, MD, USA) supplemented with 10% (v/v) FBS (Opti-Gold, GenDEPOT, Katy, Texas, TX, USA) and 1% antibiotic–antimycotic (Lonza, Basel, Belgium) at 37 °C in a humid atmosphere and 5% CO₂.
The cell number and viability were measured by using the Trypan Blue exclusion assay (Lonza, Walkersville, MD, USA). The number of cells was counted in a Malassez cell counting chamber (Marienfeld, Lauda-Königshofen, Germany).

Ha Y.N., Song S., Orlikova-Boyer B., Cerella C., Christov C., Kijjoa A, & Diederich M. (2020). Petromurin C Induces Protective Autophagy and Apoptosis in FLT3-ITD-Positive AML: Synergy with Gilteritinib. Marine Drugs, 18(1), 57.

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Generation and Validation of Mutant p53 MV4;11 Cells

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AML-193 (CRL-9589), MV4;11 (CRL-9591) and THP-1 (TIB-202) cells were obtained from American Type Culture Collection (ATCC), (Manassas, VA). THP-1 cells were cultured in RPMI culture medium supplemented with 10% fetal bovine serum (FBS), 2 mM/L L-glutamine, 25 U/mL penicillin, and 25 μg/mL streptomycin. MV4;11 cells were cultured in IMDM culture medium with supplements listed above. AML-193 cells were cultured in IMDM with 5% FBS, 0.5 ng/ml insulin, 5 ng/ml transferrin receptor and 5 ng/ml GM-CSF.
For generation of MV4;11 cells expressing mutant p53, pCMV-Neo-Bam p53 R248W (Addgene plasmid # 16437), a gift from Bert Vogelstein [11 (link)] was used. MV4;11 cells (2.5 × 10⁶) were transfected by nucleofection using Amaxa Nucleofector (Lonza, Basel, Switzerland), Kit V, program A-030. Transfected clones were isolated by single cell dilution cloning and antibiotic selection (500 μg/ml G418), followed by screening by immunoblotting.

Gopalakrishnapillai A., Kolb E.A., McCahan S.M, & Barwe S.P. (2017). Epigenetic drug combination induces remission in mouse xenograft models of pediatric acute myeloid leukemia. Leukemia research, 58, 91-97.

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FLT3-Expressing AML Cell Lines

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U-937 (expressing wild-type FLT3) and MV4-11 [expressing FLT3 with an internal tandem duplication (ITD)] human AML cell lines were purchased from the Deutsche Sammlung für Mikroorganismen und Zellkulturen, Braunschweig (DSMZ; Braunschweig, Germany). U-937 and MV4-11 cell lines were authenticated using DNA profiling with the short tandem repeat method performed by DSMZ. U-937 and MV4-11 cells were cultured in Roswell Park Memorial Institute (RPMI) 1640 medium (Lonza, Verviers, Belgium) supplemented with 10% heat-inactivated fetal calf serum (FCS; Merck Millipore, Darmstadt, Germany) and penicillin/streptomycin solution 100 U/mL (Merck Millipore, Darmstadt, Germany). All cells were maintained at 37 °C and 5% CO₂ in a humidified atmosphere.

Greco G., Schnekenburger M., Catanzaro E., Turrini E., Ferrini F., Sestili P., Diederich M, & Fimognari C. (2021). Discovery of Sulforaphane as an Inducer of Ferroptosis in U-937 Leukemia Cells: Expanding Its Anticancer Potential. Cancers, 14(1), 76.

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