Antibodies directed against EphA2 (WB analysis for total EphA2), Chlamydia Hsp60, GP96, PARP H-250, Pan Cadherin, GAPDH were purchased from Santa Cruz biotechnology. Antibodies against N-terminal EphA2 (D4A2) for WB and IF studies, pEphA2 (phospho EphA2 Ser897), pERK, pAkt, pPI3K, p85-PI3K, total Akt and total ERK were from Cell Signaling technology. Antibodies against N-terminal EphA2 (FACS, WB and IF), EphB4, PDGFRβ and IgG (control) were from R&D systems. Antibody against PDI was bought from Thermo Scientific. Anti-β-Actin and anti-Flag antibodies were purchased from Sigma-Aldrich. Phalloidin was bought from Invitrogen. Draq5 was from BioStatus Limited, UK. Polyclonal serum against IncA (anti-rabbit) was obtained through Gramsch laboratories. Inhibitors for PI3 kinase (LY294002), MEK1/2 (UO126) and EphA2 (dasatinib) were bought from Cell Signaling. Recombinant human EphA2 (rhEphA2) and recombinant human Ephrin-A1 homodimer (rhEphrin-A1 fused with IgG1-Fc) were purchased from R&D systems. Recombinant human prohibitin-His (rhPHB) also possess a His tag and is a control for rhEphA2. As a control for rhEphrin-A1, recombinant IgG1-Fc was bought from Life Technologies.
Pan cadherin
Manufactured by Santa Cruz Biotechnology
Sourced in United States
Pan-cadherin is a lab equipment product offered by Santa Cruz Biotechnology. It is a protein that is involved in the regulation of cell-cell adhesion, a fundamental process in multicellular organisms. The core function of pan-cadherin is to facilitate the formation and maintenance of cell-cell junctions.
Automatically generated - may contain errors
Lab products found in correlation
A23187, by Merck Group (2 mentions)
β actin, by Santa Cruz Biotechnology (2 mentions)
Ly294002, by Cell Signaling Technology (1 mentions)
Uo126, by Cell Signaling Technology (1 mentions)
Dasatinib, by Cell Signaling Technology (1 mentions)
Pdgfrβ, by R&D Systems (1 mentions)
Parp h 250, by Santa Cruz Biotechnology (1 mentions)
Pi3k p85, by Cell Signaling Technology (1 mentions)
P akt, by Cell Signaling Technology (1 mentions)
P pi3k, by Cell Signaling Technology (1 mentions)
Anti β actin, by Merck Group (1 mentions)
Gapdh, by Santa Cruz Biotechnology (1 mentions)
Draq5, by Biostatus (1 mentions)
Fibronectin, by Santa Cruz Biotechnology (1 mentions)
Zymogram gel, by Thermo Fisher Scientific (1 mentions)
Rapamycin, by Merck Group (1 mentions)
Phospho jnk, by Santa Cruz Biotechnology (1 mentions)
Mmp 12, by Santa Cruz Biotechnology (1 mentions)
Akt1 2 3, by Santa Cruz Biotechnology (1 mentions)
Sb203580, by Merck Group (1 mentions)
5 protocols using pan cadherin
1
Antibody-based Analysis of EphA2 Signaling
2
Regulation of hUCB-MSCs by Signaling Pathways
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hUCB-MSCs were obtained from MEDIPOST Co. Ltd (Seoul, Korea). Fetal bovine serum was bought from BioWhittaker Inc. (Walkersville, MO, USA). AA, A23187, bisindolylmaleimide I, BrdU, Indomethacin, LY294002, mitomycin C, Nordihydroguaiaretic acid (NDGA), rapamycin, 1-Aminobenzotriazole (1-ABT), and SB203580 were aquired from the Sigma Chemical Company (St Louis, MO, USA). Phospho-Aktser473, phospho-Aktthr308, Akt1/2/3, β-Actin, collagen1A, collagen3A, collagen5A, fibronectin, phospho-p38, p38, phospho-JNK, JNK, p-ERK1/2, ERK, lamin A/C, MMP-12, pan-cadherin, PKCα, PKCɛ, PKCθ, PKCζ, PKC, Phospho-PKCζ, and Sp1 antibodies were obtained from Santa Cruz Biotechnology (Santa Cruz, CA, USA). Phospho-PKC, phospho-mTORser2481 (mTORC2), phospho-mTORser2448 (mTORC1), and mTOR were purchased from Cell Signaling (Beverly, MA, USA). The Akt inhibitor I was aquired from Calbiochem (La Jolla, CA, USA). The CD34, GPR40, phospho-Sp1, and MT3-MMP antibodies were obtained from Abcam (Cambridge, MA, USA). Mithramycin A was purchased from Tocris (Bristol, UK). Zymogram gels were bought from Novex (San Diego, CA, USA). Horseradish peroxidase-conjugated goat anti-mouse and goat anti-rabbit IgG were obtained from Jackson Immunoresearch (West Grove, PA, USA). All other reagents were used as received and were of the highest purity commercially available.
3
Molecular Signaling in Cell Cultures
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Fetal bovine serum (FBS) was purchased from BioWhittaker (Walkersville, MO, USA). The following antibodies were purchased: p-PKC and PKC antibodies (Cell Signaling Technology, Danvers, MA, USA); NOX2 antibody (BD Biosciences, Franklin Lakes, NJ, USA); NCF1 antibody (LifeSpan Biosciences, Seattle, WA, USA); ITLN and Na+/K+ ATPase antibodies (abcam, Cambridge, MA, USA); p-ERK1/2, ERK, p-JNK, JNK, p-p38 MAPK, p38 MAPK, p-c-Src, c-Src, p-FAK, FAK, pan-cadherin, PKCα, PKCδ, and PKCζ antibodies (Santa Cruz Biotechnology, Paso Robles, CA, USA); Horseradish peroxidase (HRP)-conjugated goat anti-rabbit and goat anti-mouse IgG antibodies (Jackson Immunoresearch, West Grove, PA, USA); 2′, 7′-dichlorofluorescein diacetate (CM-H2DCFDA) was obtained from Invitrogen (Carlsbad, CA, USA). A23187, methyl-β-cyclodextrin (MβCD), N-acetyl-l-cysteine (NAC) and 5-azacytidine were purchased from Sigma Chemical Company (St. Louis, MO, USA). The concentrations of all of pharmacological inhibitors listed did not show any significant cytotoxic effects by themselves as confirmed by FACS analysis in each experiment. All other reagents were of the highest purity commercially available and were used as received.
4
Biochemical Analysis of Protein Trafficking
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Fetal bovine serum (#SH30088.031R) and antibiotic-antimycotic mixture (#15240062) were purchased from Hyclone (Logan, UT, USA) and Gibco (Grand Island, NY, USA), respectively. The antibodies of TGN38 (#sc-166594), VDAC1 (#sc-390996), pancadherin (#sc-59876), N-cadherin (#sc-7939), GPR (#sc-390276), VAPB (#sc-293364), PSEN1 (#sc-365450), and β-actin (#sc-47778) were purchased from Santa Cruz Biotechnology (Paso Robles, CA, USA). The antibodies of IP3R (#ab5804), TOMM20 (#ab56783), Aβ (#ab2539), APP (#ab32136), and Rab7 (#ab50533) were obtained from Abcam (Cambridge, MA, USA). The Rab5 antibody (#NB120-13253) and Rab7 antibody (#NBP1-87174) were purchased from Novus Biologicals (Littleton, CO, USA). The AICD antibody (#811901) antibody was purchased from Biolegend (San Diego, CA, USA). The Rer1 antibody was obtained from Antibodies-online (Limerick, PA, USA). The protrudin antibody (#12680-1-AP) was purchased from Proteintech group (Rosemont, IL, USA). Cortisol (#H4001), corticosterone (#C2505), DAPI (#23397), dynasore (#D7693), α-tubulin antibody (#T6074), RU 486 (#M8046), and DAPT (#D5942) were obtained from Sigma Chemical Company (St. Louis, MO, USA). The plasmids for pcDNA3.1/RER1-c-eGFP and pcDNA3.1/c-eGFP were purchased from KomaBiotech (Seoul, Korea).
5
Quantifying Duodenal Tissue Markers
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Duodenal tissue sections were fixed in formalin for 15 min (Sigma-Aldrich; Merck KGaA, Darmstadt, Germany), blocked for 1 h at room temperature with 1% BSA (Sigma-Aldrich; Merck KGaA, Darmstadt, Germany) and incubated overnight with primary antibodies anti-MUC2 (1:500) (Santa Cruz, CA, USA), pan-cadherin (1:500) (Santa Cruz, CA, USA), anti-Ki67 (1:1000) (Cell Signaling, Danvers, MA, USA) or anti-pH3 (1:1000) (Cell Signaling, Danvers, Massachusetts, USA). Appropriate fluorophore-labelled secondary antibody (1:1000) and Hoechst 33,342 [1: 20,000 dilution (Life Technology, Carlsbad, CA, USA)] were incubated for 1 h at room temperature, and coverslips mounted with Vectashield (Vector Laboratories, Burlingame, CA, USA). TUNEL staining was performed using the In Situ Cell Death Detection Fluorescein kit (Roche Diagnostics, Rotkreuz, Switzerland), according to the manufacturer’s protocol. Images were collected using a confocal laser scanning microscope (TCS SP8, Leica, Wetzlar, Germany).
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