Male athymic nude foxn1nu mice

Manufactured by Inotiv

Male athymic nude-Foxn1nu mice are laboratory mice that lack a functional thymus gland, resulting in a deficiency of T cells. They are commonly used in research due to their impaired immune system, which allows for the study of human cancer cells and other xenograft models without rejection by the host's immune system.

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Lab products found in correlation

Ir 780 dye, by Merck Group (1 mentions) Odyssey clx, by LI COR (1 mentions)

2 protocols using male athymic nude foxn1nu mice

Islet Transplantation in Nude Mice

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Male athymic nude-Foxn1^nu mice (Envigo, Horst, The Netherlands) housed under specific pathogen-free conditions on a 12-h light cycle with free access to food and water were used as recipients of human islet transplantation. Mice were maintained in the animal facility of the Bellvitge Biomedical Research Institute (IDIBELL) following to the guidelines and recommendations of the Association for Assessment and Accreditation of Laboratory Animal Care International (AAALAC International, accreditation number 1155). Mice were sacrificed by pentobarbital overdose. Animal experimental procedures were reviewed and approved by the Ethical Committee for Animal Experimentation of the IDIBELL (identification number DAAM 4588).

Marín-Cañas S., Estil·les E., Llado L., San José P., Nacher M., Téllez N, & Montanya E. (2019). Pancreatic ductal cells may have a negative effect on human islet transplantation. PLoS ONE, 14(7), e0220064.

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In vivo Bone Homing Nanoparticle Tracking

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After institutional IACUC approval, in vivo bone homing experiment was performed. Male athymic nude-foxn1nu mice were purchased from Envigo. Mice were allowed to acclimate 1 week before experiments. Treatment group mice were injected intravenously with 100 μL of freshly prepared saline, dye, BiNPs, or P-BiNPs via lateral tail vein injection. Treatment groups were prepared as described above with incorporation of IR-780 dye (Sigma-Aldrich, USA) into the core of the nanoparticle prior to coating or the equivalent concentration of dye used in dye only group to ensure consistency among groups. Two hours after injection, mice were sacrificed, organs were excised, and NIR emission was imaged and quantified with Odyssey CLx (LI-COR, USA). The following settings for Odyssey CLx for imaging were used: resolution, 169 nm; focus offset, 2 mm; channel, 800 nm; intensity, L2; exposure time, 7 min. The software used for this analysis was Image Studio 1.0.20.

Gdowski A.S., Lampe J.B., Lin V.J., Joshi R., Wang Y.C., Mukerjee A., Vishwanatha J.K, & Ranjan A.P. (2019). Bioinspired Nanoparticles Engineered for Enhanced Delivery to the Bone. ACS applied nano materials, 2(10), 6249-6257.

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