Brahms pct kit

Serum procalcitonin, CRP and WBC count were measured in the first day of admission to inpatient clinics. WBC level was determined with the Sysmex XP-300(®) kit. Hematology Analyzer (Sysmex, America, Inc.) CRP was measured using the immunoturbidimetric assay. Pars Azmin, Tehran, Iran) Procalcitonin value was measured with BRAHMS PCT kit (Roche Diagnostic, Mannheim, Germany). A serum level equal to or greater than 2 ng / mL was considered positive for CA^{(7 (link))}.
We collected 5 cm³ of maternal serum after 12 hours of fasting on the first day of hospitalization in a biochemistry tube. Then, we centrifuged it at 1000 rpm and 2 °C-8 °C temperature for 15 minutes. The supernatant of the serum was placed in 1.5 mL Eppendorf tubes and stored at -80 °C. Maternal serum plasma PARK 7 level was measured using the ELISA kit, following the manufacturer’s instructions (Recombinant Human PARK7/DJ-1 Protein (His Tag); catalog no: PKSH030826, Cusabio Biotech Co. Ltd. China).

Blood tests for measuring peripheral WBC counts, and CRP, LDH, and mycoplasma IgM antibody levels were performed on admission. A blood test for measuring mycoplasma IgM antibody level was performed within 7 days after admission for diagnosing MPP.
A serum sample for PCT level determination was collected on admission and stored at -20°C until analyzed. Serum PCT level was measured on a Roche Cobas e601 analyzer based on an electrochemiluminescence immunoassay using the Elecsys BRAHMS PCT kit (Roche Diagnostics Korea, Seoul, Korea). The assay detection range was 0.02–100 ng/mL.
Serum mycoplasma IgM level was assayed using an enzyme immunoassay (RIDASCREEN; R-Biopharm AG, Darmstadt, Germany). The cutoff level for mycoplasma IgM antibody level was 71 U/mL. Mycoplasma PCR of sputum or nasopharyngeal aspiration samples was performed using the Bio-Core M. pneumoniae PCR Kit (Seegene, Seoul, Korea). The mycoplasma PCR was performed in 2 patients, and those patients showed positive results in both PCR and serology tests. PCR was not performed in the remaining patients, and the diagnosis was established using the enzyme immunoassay (EIA) method.