Salmonella enterica serovar Typhimurium (ATCC 14028) (ST) was used for this study. Luria-Bertani (LB) (Becton, Dickinson and Co., Franklin Lakes, NJ, USA) agar and broth were used as the medium on which ST was grown, and it was incubated at 37 °C under aerobic conditions (Thermo Fisher Scientific Inc., Waltham, MA, USA). XLD (Hardy diagnostics, Santa Maria, CA, USA) and SS (Becton, Dickinson and Co., Franklin Lakes, NJ, USA) agar were used as differential media that allowed for the identification and differentiation of ST from other gram-negative coliforms.
Xylose lysine deoxycholate (xld)
Manufactured by Hardy Diagnostics
Sourced in United States, India
XLD is a selective and differential culture medium used for the isolation and identification of Salmonella and Shigella species. It contains lactose, sucrose, and xylose as carbohydrate sources, along with a pH indicator that detects acid production. Colonies of Salmonella and Shigella appear red with black centers due to hydrogen sulfide production.
Automatically generated - may contain errors
2 protocols using xylose lysine deoxycholate (xld)
1
Salmonella Typhimurium Growth and Identification
2
Isolation of Enteric Bacterial Pathogens
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A loopful of stool sample was inoculated into alkaline peptone water; pH 8.2 and incubated for 8 hours at 37 o C. Subculture was done on TCBS (Central drug house ltd, India), XLD (Hardy diagnostics, USA) and Mackonkey (Oxoid, UK) and further incubated at 37 o C for 24 hours. Direct culture was carried out on TCBS, XLD, Campylobacter blood free agar (Oxoid, UK), and enrichment in Selenite F medium (Himedia laboratories PVT ltd India) to isolate the bacterial pathogens. Rectal swabs were processed in the same manner.
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