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Aldh1

Manufactured by Takara Bio
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Sourced in Japan

Aldh1 is a laboratory product used for the detection and analysis of aldehyde dehydrogenase 1, an enzyme involved in the metabolism of alcohol and other aldehydes. The core function of Aldh1 is to provide a reliable method for measuring the activity and expression of this enzyme in biological samples.

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Lab products found in correlation

Revertra ace qpcr rt kit, by Toyobo (1 mentions) Light cycler 480 sybr system, by Roche (1 mentions) Abi prism 7300 sequence detector, by Thermo Fisher Scientific (1 mentions) Primescript rt reagent kit, by Takara Bio (1 mentions) Rnaiso plus, by Takara Bio (1 mentions) Sybr green fluorescence, by Takara Bio (1 mentions)

2 protocols using aldh1

1

Quantification of Hepatic Gene Expression

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Total RNA isolated from liver samples was used. The cDNA synthesis of total RNA was carried out using a ReverTra Ace qPCR RT kit (TOYOBO, Osaka, Japan) according to the manufacturer’s instructions. To measure the mRNA amount in liver samples, RT-PCR was conducted with SYBR green dye using the LightCycler 480 SYBR system (Roche Applied Science, Mannheim, Germany). The oligonucleotide primers used for amplification were obtained from commercial products (Adh1, Aldh1, Aldh2, Gsta2, Gsta4, Gstm1, Gstt2, Sod1 and Actb (β-actin) (Takara Bio, Shiga, Japan)). The expression of each gene was normalized to that of β-actin mRNA.
Takase T., Toyoda T., Kobayashi N., Inoue T., Ishijima T., Abe K., Kinoshita H., Tsuchiya Y, & Okada S. (2021). Dietary iso-α-acids prevent acetaldehyde-induced liver injury through Nrf2-mediated gene expression. PLoS ONE, 16(2), e0246327.
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2

Stem Cell Marker Expression Analysis

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Total RNA was isolated using an RNAiso Plus (Takara Bio Inc., Otsu, Japan). RNA was reverse-transcribed using a PrimeScript RT Reagent Kit (Takara Bio Inc.). Quantitative real-time PCR was performed using TaqMan polymerase with SYBR Green Fluorescence (Takara Bio Inc.) and an ABI PRISM 7300 Sequence Detector (Applied Biosystems, Foster City, CA, USA). The relative expression level was determined using the 2−ΔΔCq normalization method. Predesigned primer sets for Nanog, Sox2, ALDH1, Oct3/4, Bmi1, and ABCG2 were purchased from Takara Bio Inc. (Perfect Real Time PCR support system).
Hide T., Komohara Y., Miyasato Y., Nakamura H., Makino K., Takeya M., Kuratsu J.I., Mukasa A, & Yano S. (2018). Oligodendrocyte Progenitor Cells and Macrophages/Microglia Produce Glioma Stem Cell Niches at the Tumor Border. EBioMedicine, 30, 94-104.
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