Annexin V/PI apoptosis-detection kit (Nanjing KeyGen Biotech Co., Ltd.) was used to evaluate the apoptosis according to the manufacturer’s protocol. The stained cells were immediately examined using an EPICS XL-MCL FACScan flow cytometer (Beckman Coulter, Inc., Brea, CA, USA) and analyzed using FlowJo 8.7.1 software (Ashland, OR). The results showed healthy viable cells in the lower left quadrant (Q4) on the scatter plot as (FITC-/PI-). The lower right quadrant (Q3) represented the early stage apoptotic cells as (FITC + /PI-). The upper right quadrant (Q2) represented necrotic cells and late stage apoptotic cells as (FITC + /PI +). Apoptotic rate = percentage of early stage apoptotic cells (Q3) + percentage of late stage apoptotic cells (Q2) [15 (link)].
Epics xl mcl facscan flow cytometer
Manufactured by Beckman Coulter
Sourced in United States
The EPICS XL-MCL FACScan flow cytometer is a compact, bench-top instrument designed for multiparametric analysis of cells or particles in suspension. It utilizes a solid-state laser and advanced optics to provide high-performance flow cytometry capabilities.
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2 protocols using epics xl mcl facscan flow cytometer
1
Apoptosis Quantification Using Flow Cytometry
2
Annexin V-FITC Apoptosis Assay
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Cell apoptosis was monitored using the Annexin V-FITC Apoptosis Detection kit (Beyotime Institute of Biotechnology). Briefly, a total of 5×104 HUVECs were collected at 48 h post-transfection and resuspended in cold phosphate-buffered saline (PBS). Cells were then collected and resuspended in 195 µl Annexin V-FITC binding buffer followed by the addition of 5 µl Annexin V-FITC and 10 µl PI. Cells were incubated at room temperature for 20 min in the dark and subsequently analyzed using an EPICS XL-MCL FACScan flow cytometer (Beckman Coulter, Inc.).
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