Pifithrin μ

Glucose oxidase, Cyclosporine A, Sanglifehrin A, DCFH-DA, DAPI, calcein-AM and Rhodamine-123 were purchased from the Sigma Chemical Corp (St. Louis, USA). The molecular probes including MitoSOX™, Mito-Tracker-Red/Green were obtained from Invitrogen Corporation. (Carlsbad, USA). The Malondialdehyde assay kits were purchased from Beyotime Institute of Biotechnology (Nanjing, CHINA). The Annexin V-FITC/Propidium iodide apoptosis kit, TUNEL apoptosis detection kit, Mitochondria Fractionation Kit, and Nucleus Fractionation Kit was purchased from the Roche (Cambridge, USA). GAPDH, COX IV, Bax, Bcl-2, cytochrome C antibodies, Pifithrin-α, Pifithrin-μ and ionomycin were obtained from Santa Cruz Biotechnology (Santa Cruz, USA). P53 antibodies, Goat anti-Rabbit IgG-FITC, and Goat anti-Rabbit IgG-(H+L)-HRP were purchased from the abcam (Cambridge, USA). Caspase-9 and Caspase-3 Colorimetric Assay Kit was obtained from Keygen (Nanjing, China). All other chemicals and solvents were of analytical grade with highest purity commercially available.