Triciribine

Manufactured by Cayman Chemical

Sourced in United States

Triciribine is a chemical compound used in research laboratories. It functions as an inhibitor of the phosphorylation of AKT, a protein kinase involved in various cellular processes. The primary use of Triciribine is for research purposes, without interpretation of its intended applications.

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3 protocols using triciribine

Neuronal Oxidative Stress Analysis

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Dulbecco’s modified Eagle’s medium (DMEM) was purchased from Sigma Chemical (St. Louis, MO, USA). Penicillin–streptomycin solution was purchased from FUJIFILM Wako Pure Chemical Corp. (Osaka, Japan). Fetal bovine serum (FBS), neurobasal medium (phenol red and glutamine minus), and B27 supplement were obtained from Thermo Fischer Scientific (Waltham, MA, USA). Bexarotene and triciribine were obtained from Cayman Chemicals (Ann Arbor, MI, USA). UVI3003 was purchased from Tocris Bioscience (Bristol, UK). Methylmercury chloride was obtained from Tokyo Chemical Industry (Tokyo, Japan). Carboxy-H₂DCFDA was obtained from Molecular Probes (Eugene, OR, USA).

Oguro A., Fujita K., Ishihara Y., Yamamoto M, & Yamazaki T. (2021). DHA and Its Metabolites Have a Protective Role against Methylmercury-Induced Neurotoxicity in Mouse Primary Neuron and SH-SY5Y Cells. International Journal of Molecular Sciences, 22(6), 3213.

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Cell Culture Media and Supplements

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Cell culture media and supplements such as RPMI 1640, DMEM, PBS, and antibiotics (penicillin, streptomycin, and puromycin) were purchased from Lonza (Allendale, NJ), Fisher Scientific (Pittsburgh, PA) or Santa Cruz Biotechnology (Dallas, TX). Heat-inactivated fetal bovine serum (FBS) was obtained from Atlanta Biologicals (Flowery Branch, GA). Sodium butyrate and sanguinarine were procured from Sigma-Aldrich (St. Louis, MO). Trichostatin A (TSA), apicidin, HC toxin, LY294002, PX866, CAL-101, MK2206, Triciribine, GDC0068, Rapamycin, AZD8055, and BEZ235 were obtained from Cayman Chemical (Ann Arbor, MI). Tetrandrine was acquired from Santa Cruz Biotechnology, and (–)-depudecin was purchased from BioVision (Milpitas, CA) and MyBioSource (San Diego, CA). Datiscetin was ordered from BOC Sciences (Shirley, NY), while wortmannin and CUDC-907 were procured from Selleck Chemicals (Houston, TX).

Lyu W., Deng Z., Sunkara L.T., Becker S., Robinson K., Matts R, & Zhang G. (2018). High Throughput Screening for Natural Host Defense Peptide-Inducing Compounds as Novel Alternatives to Antibiotics. Frontiers in Cellular and Infection Microbiology, 8, 191.

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Modulating B and T Cell Proliferation

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Purified naive B cells labeled with CFSE, CTV, or eFluor670 cell proliferation dyes were cultured in lipopolysaccharide (LPS-SM ultrapure; Invivogen; 10–25 μg/mL) in 48 well plates at 2.5 × 10⁵ cells/mL of culture media in a 37 °C humidified environment with 5% CO₂. To activate CD8⁺ T cell in vitro, wild-type or P14⁺ CD8⁺ T cells were purified, labeled with cell proliferation dye and stimulated with immobilized anti-CD3 (5 μg/ml), soluble anti-CD28 (1 μg/ml) and recombinant IL-2 (30 IU), or by gp33 (1 μg/ml; Anaspec) peptide-loaded splenocytes, respectively. Memory CD8⁺ T cells were harvested at 120⁺ days after LCMV infection.
Small molecule inhibitors of PI3K (LY294002; Cell Signaling), pan-AKT (Triciribine; Cayman Chemical Company), mTOR (Rapamycin; Selleckchem), and FoxO1 (AS1842856; Calbiochem) were added to the cells at the time of activation. Mitochondria membrane potential was measured by incubating cells with MitoTracker CMXRos red-fluorescent dye (Cell Signaling) for 30 minutes followed by washing cells three times with ice-cold culture media.

Lin W.H., Adams W.C., Nish S.A., Chen Y.H., Yen B., Rothman N.J., Kratchmarov R., Okada T., Klein U, & Reiner S.L. (2015). Asymmetric PI3K Signaling Driving Developmental and Regenerative Cell Fate Bifurcation. Cell reports, 13(10), 2203-2218.

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