Radioimmunoprecipitation assay ripa lysis and extraction buffers

Manufactured by Thermo Fisher Scientific

Radioimmunoprecipitation assay (RIPA) lysis and extraction buffers are aqueous solutions used to extract and solubilize proteins from cells and tissues. They are designed to preserve the native conformation and interactions of proteins while disrupting cellular and nuclear membranes.

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Lab products found in correlation

Trizol reagent, by Thermo Fisher Scientific (2 mentions) Anti rabbit igg h l hrp conjugated antibody, by Promega (1 mentions) Cd9 antibody, by Cell Signaling Technology (1 mentions) Alexa fluor 488 goat anti rabbit igg h l secondary antibody, by Thermo Fisher Scientific (1 mentions) Goat anti mouse igg horseradish peroxidase hrp conjugated antibody, by Thermo Fisher Scientific (1 mentions) 4 6 diamidino 2 phenylindole (dapi), by Thermo Fisher Scientific (1 mentions) Gapdh antibody, by Thermo Fisher Scientific (1 mentions) Dapi 4 6 diamidino 2 phenylindole, by Thermo Fisher Scientific (1 mentions) Horseradish peroxidase hrp conjugated affinipure goat anti rabbit igg h l, by Proteintech (1 mentions) Anti gapdh rabbit monoclonal antibody 14c10, by Cell Signaling Technology (1 mentions) Anti β actin 13e5 rabbit monoclonal antibody, by Cell Signaling Technology (1 mentions)

Close spelling variants of this product

Radioimmunoprecipitation assay lysis and extraction buffer RIPA Lysis and Extraction Buffer RIPA radioimmunoprecipitation assay buffer RIPA lysis extraction buffer Radioimmunoprecipitation assay lysis buffer Lysis and Extraction Buffer Radioimmunoprecipitation (RIPA) buffer Radioimmunoprecipitation lysis buffer RIPA lysis buffer RIPA extraction buffer

2 protocols using radioimmunoprecipitation assay ripa lysis and extraction buffers

Phosphorylation-Specific Antibody Assay

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TAK1 (phosphor-Ser192) (#orb7051) and NFKB1 (phosphor-Ser933) antibodies were purchased from Biorbyt (Cambridge, UK). The Anti-NF-kappaB p105 (pS932) phospho antibody (#MBS8210747) was purchased from MyBioSource (San Diego, CA). Mouse anti-chicken glyceraldehyde-3-phosphate dehydrogenase (GAPDH) antibody (#AM4300), goat anti-mouse IgG horseradish peroxidase (HRP)-conjugated antibody (#A16078), and radioimmunoprecipitation assay (RIPA) lysis and extraction buffers were purchased from Thermo Fisher Scientific (Waltham, MA). Alexa Fluor 488 goat anti-rabbit IgG (H+L) secondary antibody and 4,6-diamidino-2-phenylindole (DAPI) were purchased from Invitrogen (#A-11008; Carlsbad, CA). Anti-rabbit IgG (H+L) HRP-conjugated antibody was purchased from Promega (#W4011; Madison, WI). The CD9 antibody was purchased from Cell Signaling (#13174; Danvers, MA).

Hong Y., Lee J., Vu T.H., Lee S., Lillehoj H.S, & Hong Y.H. (2021). Immunomodulatory effects of poly(I:C)-stimulated exosomes derived from chicken macrophages. Poultry Science, 100(8), 101247.

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ASFV Protein Detection Protocols

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For WB analysis, anti-p30, -p72, anti-pC315R, and anti-pH359L rabbit sera were raised against recombinant ASFV p30, p72 pC315R, and pH359L proteins and deposited at the African Swine Fever Regional Laboratory (Lanzhou), LVRI, of the Chinese Academy of Agricultural Sciences. Anti-CD2v mouse sera were kindly provided by Liguo Zhang from Institute of Biophysics, Chinese Academy of Sciences. Anti-β-actin (13E5) rabbit monoclonal antibody (catalog no. 4970) and anti-GAPDH (14C10) rabbit monoclonal antibody (catalog no. 2118) were purchased from Cell Signaling Technology. Anti-β-tubulin rabbit polyclonal antibody (catalog no. 10094-1-AP) and horseradish peroxidase (HRP)-conjugated AffiniPure goat anti-rabbit IgG(H+L) (catalog no. SA00001-2) were purchased from ProteinTech Group. Fluorescein isothiocyanate (FITC)-conjugated goat anti-rabbit IgG secondary antibody (catalog no. F0382) was purchased from Sigma-Aldrich. A Cell Counting Kit-8 (CCK-8; catalog no. K1018-30) was purchased from APExBIO (USA). TRIzol reagent (catalog no. 15596018), DAPI (4′,6′-diamidino-2-phenylindole; catalog no. 62248), octadecyl rhodamine B (R18, catalog no. O246), and radioimmunoprecipitation assay (RIPA) lysis and extraction buffers (catalog no. 89901) were purchased from Thermo Fisher Scientific. Filters (0.22-μm pore size) were purchased from Millipore.

Zhao Y., Niu Q., Yang S., Yang J., Zhang Z., Geng S., Fan J., Liu Z., Guan G., Liu Z., Zhou J., Hu H., Luo J, & Yin H. (2022). Inhibition of BET Family Proteins Suppresses African Swine Fever Virus Infection. Microbiology Spectrum, 10(4), e02419-21.

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