Cell counting and cell viability were performed to determine the cell growth and viability of ADSCs encapsulated in Col-Tgels by using a cell counter and a Cell Counting Kit-8 (CCK-8, Dojindo Molecular Technologies, Rockville, MD), as described18 (link). Briefly, cells were released from Col-Tgel-Cell constructs using 20 units of bacterial collagenase type II (Worthington, Lakewood, NJ) prior to counting by a cell counting machine (Beckman Coulter, Brea, CA). For the CCK-8 assay, 500 μL of the CCK working solution (CCK-8 stock solution in growth medium, 1:40) was directly applied to the Col-Tgel-Cell construct for 4 h. Subsequently, 100 μL of the supernatant was transferred into 96-well plates and the absorbance was measured in a multiplate reader at 450 nm (Molecular Devices, Sunnyvale, CA).
Cell counting machine
Manufactured by Beckman Coulter
Sourced in China
The cell counting machine is a laboratory instrument used to determine the number of cells in a sample. It provides an automated and standardized method for counting cells, which is essential for various applications in cell biology, tissue engineering, and biomedical research.
Automatically generated - may contain errors
2 protocols using cell counting machine
1
Cell Viability Assay for ADSC-Encapsulated Col-Tgel
2
Blood Cell Counting Protocol
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
One milliliter of pre- and post-enrichment samples from every patient was treated with red blood cell lysis buffer (BioTime, Shanghai, China) and was loaded on to the cell-counting machine (Beckman Coulter, Brea, California, USA) for karyocyte counting and cell viability testing.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!