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Recombinant human wnt5a protein

Manufactured by R&D Systems
Sourced in China

Recombinant human Wnt5a protein is a laboratory reagent produced using recombinant DNA technology. Wnt5a is a signaling protein that plays a role in various cellular processes. The core function of this protein is to facilitate the study of Wnt5a-related biological mechanisms in research applications.

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2 protocols using recombinant human wnt5a protein

1

Wnt Signaling Modulation in Cellular Assays

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Arecoline (#S2614, Selleck Chemicals), Recombinant human Wnt1 protein (#ab84080, Abcam plc.), Recombinant human Wnt2 protein (#H00007472-P01, Bio-Techne China Co. Ltd.), Recombinant human Wnt5a protein (#645-WN-010, R&D system, Wiesbaden-Nordenstadt, Germany), Mithramycin A (Sigma), Chromomycin A3 (Sigma) and antibodies were used at the indicated concentrations and time points. Lipofectamine LTX (#15338100, Invitrogen) and Lipofectamine RNAiMAX (#13778150, Invitrogen) were used for transient gene or siRNA transfection of cells. The following primary antibodies were used: Wnt1 (#ab15251), Wnt2 (#ab109222), Wnt5a (#ab179824) and GAPDH (#ab181602) were from Abcam plc..
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2

Wnt5a Protein Localization Assay

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Recombinant human Wnt5a protein was purchased from R&D systems; anti-Wnt5a was from Abcam; anti-Coro1A and anti-NFATc2 were from Affinity; anti-Lamin B1 was from Origene; Mitotracker Red, phalloidin FITC, and fluo-4 were from Molecular Probes.
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