Glyceraldehyde 3 phosphate dehydrogenase gapdh antibody

Manufactured by Biosynth

Sourced in United States

Glyceraldehyde 3-phosphate dehydrogenase (GAPDH) antibody is a laboratory reagent used to detect the presence and quantify the GAPDH protein in biological samples. GAPDH is an enzyme involved in the glycolysis pathway and is commonly used as a reference or housekeeping gene for analyzing gene expression levels.

Automatically generated - may contain errors

Lab products found in correlation

Multi gauge v3, by Fujifilm (1 mentions) Fura 2 am, by Thermo Fisher Scientific (1 mentions) Rp camps, by Bio-Techne (1 mentions) Phospho specific antibody, by Cell Signaling Technology (1 mentions) Pertussis toxin ptx, by Bio-Techne (1 mentions) Baycyslt2, by Cayman Chemical (1 mentions) L 161, by Cayman Chemical (1 mentions) Iloprost, by Cayman Chemical (1 mentions) Sulprostone, by Cayman Chemical (1 mentions) Kt5823, by Bio-Techne (1 mentions) Mk571, by Cayman Chemical (1 mentions) Gf109203x, by Bio-Techne (1 mentions) Pd98059, by Bio-Techne (1 mentions) Butaprost, by Cayman Chemical (1 mentions)

Spelling variants of this product

GAPDH (73 citations) GAPDH antibody (2 citations)

2 protocols using glyceraldehyde 3 phosphate dehydrogenase gapdh antibody

HDAC2 Activity Quantification by Western Blot

Check if the same lab product or an alternative is used in the 5 most similar protocols

HDAC2 activity was measured by Western blot analysis in 10 rats per group. Briefly, small quick-frozen bronchi were individually homogenised on dry ice and boiled in 50 μl of sodium dodecyl sulfate (SDS) sample buffer (1.5% dithiothreitol, 2% SDS, 80 mM Tris-HCl (pH 6.8), 10% glycerol and 0.01% bromophenol blue) for 5 min and then separated by SDS polyacrylamide gel electrophoresis. Antibody against HDAC2 was purchased from Santa Cruz Biotechnology (Texas, USA). Glyceraldehyde 3-phosphate dehydrogenase (GAPDH) antibody was purchased from Fitzgerald Industries International (Massachusetts, USA). The levels of proteins in the immunoblots were quantified by scanning densitometry (Fuji Multigauge V.3.0 Software). The luminescent signals from all immunoblots were within the linear range.

Li J., Wu S., Tang H., Huang W., Wang L., Zhou H., Zhou M., Wang H, & Li J. (2015). Long-term effects of acupuncture treatment on airway smooth muscle in a rat model of smoke-induced chronic obstructive pulmonary disease. Acupuncture in Medicine, 34(2), 107-113.

+ Open protocol

+ Expand

Characterization of Inflammatory Mediator Signaling

Check if the same lab product or an alternative is used in the 5 most similar protocols

LTD₄, PGE₂, MK571, BayCysLT₂, iloprost, butaprost, sulprostone, L-798, ONO-871, L-161, and PGD₂ ELISA kits were purchased from Cayman Chemicals (Ann Arbor, Mich). KT5823, PD98059, pertussis toxin (PTX), H7, GF109203X, Rp-cAMPS, and H89 inhibitors were from Tocris Bioscience (Minneapolis, Minn). Fura-2 AM was from Molecular Probes (Eugene, Ore), phospho-specific antibodies were from Cell Signaling Technology (Danvers, Mass), and glyceraldehyde-3-phosphate dehydrogenase (GAPDH) antibody was from Fitzgerald (Acton, Mass). All secondary antibodies were obtained from Jackson ImmunoResearch (West Grove, Pa). Nonspecific small interfering RNA (siRNA) and isoform-specific siRNAs for CysLT₁R, EP₃, and PKG were obtained from Dharmacon (Lafayette, Colo), and the macrophage inflammatory protein 1β (MIP-1β) ELISA kit was from R&D Systems (Minneapolis, Minn). Cytokines for hMC cultures were obtained from PeproTech (Rocky Hill, NJ).

Kondeti V., Al-Azzam N., Duah E., Thodeti C.K., Boyce J.A, & Paruchuri S. (2015). Leukotriene D4 and prostaglandin E2 signals synergize and potentiate vascular inflammation in a mast cell–dependent manner through cysteinyl leukotriene receptor 1 and E-prostanoid receptor 3. The Journal of allergy and clinical immunology, 137(1), 289-298.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!