The miR-20a-5p mimic, miR-20b-5p mimic, mimic control duplexes, miR-20a-5p inhibitor, miR-20b-5p inhibitor and inhibitor NC were purchased from RiboBio. siRNA against E2F1 was purchased from GenePharma. Lipofectamine 3000 reagent (Invitrogen) was used for transfection according to the manufacturer’s instructions. 50 nM miRNA mimics, 100 nM miRNA inhibitor (as well as the mixed inhibitors) or 100 nM siRNA was used in the transfection assay.
Mir 20a 5p mimic
Manufactured by RiboBio
Sourced in China
The MiR-20a-5p mimic is a synthetic, double-stranded RNA molecule designed to mimic the function of the endogenous miR-20a-5p microRNA. MicroRNAs are small, non-coding RNA molecules that play important roles in regulating gene expression. The MiR-20a-5p mimic can be used in research applications to study the biological functions and regulatory mechanisms of miR-20a-5p.
Automatically generated - may contain errors
Lab products found in correlation
Mir 20a 5p inhibitor, by RiboBio (2 mentions)
Inhibitor nc, by RiboBio (1 mentions)
Mimic control duplexes, by RiboBio (1 mentions)
Lipofectamine 3000 reagent, by Thermo Fisher Scientific (1 mentions)
Mir 20b 5p mimic, by RiboBio (1 mentions)
Mir 20b 5p inhibitor, by RiboBio (1 mentions)
Sirna against e2f1, by GenePharma (1 mentions)
Mir nc mimic, by RiboBio (1 mentions)
Pcd cir empty vector, by Vector Laboratories (1 mentions)
Lipofectamine 3000 kit, by Thermo Fisher Scientific (1 mentions)
Sirock2, by RiboBio (1 mentions)
Ta cloning kit, by Thermo Fisher Scientific (1 mentions)
Thp 1, by American Type Culture Collection (1 mentions)
Mv4 11, by American Type Culture Collection (1 mentions)
Molm 13, by American Type Culture Collection (1 mentions)
Lipofectamine 2000 reagent, by Thermo Fisher Scientific (1 mentions)
Pcdna3.1 vector, by Thermo Fisher Scientific (1 mentions)
Pentr tm u6 plasmid, by GenePharma (1 mentions)
Mir nc, by RiboBio (1 mentions)
Sh nc, by GenePharma (1 mentions)
4 protocols using mir 20a 5p mimic
1
Investigating miRNA-mediated Gene Regulation
2
Silencing circUSP36 and ROCK2 via siRNA
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Small interfering RNA (siRNA) targeting circUSP36 (si-circUSP36: 5′-GGG GAC CGC ATG GGG CTG TGT-3′) or ROCK2 (si-ROCK2: 5′-CAG AAG TGC AAG TCT ATT A-3′) for circUSP36 knockdown or ROCK2 knockdown and their corresponding controls (si-NC; scramble) were synthesized by Guangzhou RiboBio Co., Ltd. The mimic for miR-20a-5p for the restoration of miR-20a-5p expression (miR-20a-5p mimic), the inhibitor of miR-20a-5p for the inhibition of miR-20a-5p expression (miR-20a-5p inhibitor), and their corresponding controls (miR-NC mimic or miR-NC inhibitor; scramble) were purchased from Guangzhou RiboBio Co., Ltd. CircUSP36 was assembled into the pCD-ciR vector for circUSP36 overexpression (oe-circUSP36) (GenePharma, Inc.), and pCD-ciR empty vector (Vector) was used as its control. Lipofectamine 3000 kit (Invitrogen; Thermo Fisher Scientific, Inc.) was applied to perform cell transfection (siRNAs, 30 nM; miRNA mimic or inhibitor, 40 nM; vector, 1.5 µg) according to the manufacturer's instructions.
3
Investigating PSMA3-AS1 in FLT3-ITD+ Cell Lines
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Human cell line Molm-13 (FLT3-ITD+ cell line), MV4–11 (FLT3-ITD+ cell line), THP-1 (FLT-ITD- cell line) and U937 (FLT-ITD- cell line) were obtained from ATCC (Manassas, VA, USA) and was cultured in RPMI 1640. Medium supplemented was with 10% FBS (fetal bovine serum) storing at 37°C with 5% CO2.
The cDNA of PSMA3-AS1 was synthesized according to the sequence obtained from lncBase database, and then cloned into pcDNA3.1 plasmid using TA Cloning™ Kit (Thermo Fisher Scientific). PcDNA3.1 plasmid vectors, pcDNA3.1-PSMA3-AS1 (oe-PSMA3-AS1), siRNA- PSMA3-AS1 (si-PSMA3-AS1), pcDNA3.1-ATG16L1 was obtained from Genechem (Shanghai, China). MiR-20a-5p mimics and miRNA negative control were synthesized by RiBoBio (Guangzhou, China). Transfection of blank plasmid was applied to the control group (Ctrls). Cells were transiently transfected by Lipofectamine 2000 reagent (Invitrogen) on the basis of the manufacturer’s protocol. After being transfected for 24 h, cells were obtained and used for experiments.
The cDNA of PSMA3-AS1 was synthesized according to the sequence obtained from lncBase database, and then cloned into pcDNA3.1 plasmid using TA Cloning™ Kit (Thermo Fisher Scientific). PcDNA3.1 plasmid vectors, pcDNA3.1-PSMA3-AS1 (oe-PSMA3-AS1), siRNA- PSMA3-AS1 (si-PSMA3-AS1), pcDNA3.1-ATG16L1 was obtained from Genechem (Shanghai, China). MiR-20a-5p mimics and miRNA negative control were synthesized by RiBoBio (Guangzhou, China). Transfection of blank plasmid was applied to the control group (Ctrls). Cells were transiently transfected by Lipofectamine 2000 reagent (Invitrogen) on the basis of the manufacturer’s protocol. After being transfected for 24 h, cells were obtained and used for experiments.
4
Modulating lncRNA LSINCT5 and miR-20a-5p in Osteosarcoma
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The specific short hairpin RNA (shRNA) directed against human lncRNA LSINCT5 was cloned into pENTR TM/U6 plasmid (GenePharma, Shanghai, China) and called sh-LSINCT5. Non-targeted shRNA (sh-NC, GenePharma) was applied as a negative control. The full-length sequence of lncRNA LSINCT5 was transfected into pcDNA-3.1 vector (ThermoFisher Scientific, China) and called pcDNA-LSINCT5. An empty pcDNA vector was used as a negative control. miR-20a-5p mimics (miR-20a-5p-mimics), inhibitors (miR-20a-5p-inhibit), or corresponding perturbation controls (miR-NC) were synthesized by RiboBio (Guangzhou, China). XIAP-specific siRNA (si-XIAP) and siRNA negative pair (si-NC) were from Santa Cruz (United States). For overexpression of XIAP, the full-length XIAP sequence was transfected into a pDNA-3.1 vector (ThermoFisher Scientific) and called pcDNA-XIAP. pDNA-3.1 was use as blank control. On the basis of the manufacturer’s plan, all transfection was processed by Lipofectamine 3000 reagent. Stable transfected MG-63 and U2OS cells were selected by a medium incorporating 0.5 mg/mL G418 (Sigma-Aldrich, St. Louis, MO, USA). Stable transfected cells were selected for succeeding tests.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!