Horseradish peroxidase hrp conjugated polymer anti rabbit and anti mouse antibodies

The primary antibodies, rabbit anti‐human E‐cadherin monoclonal antibody (#3195) and rabbit anti‐human β‐actin monoclonal antibody (#4967), were purchased from Cell Signaling Technology Inc. Rabbit anti‐human ZEB1 antibody (HPA027524) was purchased from Sigma‐Aldrich. Mouse anti‐human HIF‐1α monoclonal antibody (ab16066), rabbit anti‐human TGF‐β antibody (ab92486), and mouse anti‐vimentin antibody (ab8978) were purchased from abcam. The secondary antibodies, horseradish peroxidase (HRP)‐conjugated polymer anti‐rabbit and anti‐mouse antibodies, were purchased from DAKO‐Agilent Technologies Co. HRP‐linked anti‐rabbit and ‐mouse antibodies were purchased from Cell Signaling Technology Inc. Alexa Fluor 594‐conjugated goat anti‐rabbit IgG and Alexa Fluor 488‐conjugated goat anti‐mouse IgG antibodies were purchased from Thermo Fisher Scientific.

For the immunohisto- and cyto-chemistry and wester blotting analyses, the following antibodies were used. The primary antibodies; rabbit anti-human PKM2 monoclonal antibody (#4053), rabbit anti-human E-cadherin monoclonal antibody (#3195), rabbit anti-human N-cadherin polyclonal antibody (#4061), rabbit anti-human ubiquitin polyclonal antibody (#3933), rabbit anti-human histone H3 polyclonal antibody (#9715), rabbit anti-human GAPDH monoclonal antibody (#2118) and mouse anti-human β-actin monoclonal antibody (#3700) were purchased from Cell Signaling Technology Inc. (Danvers, MA, USA). Rabbit anti-human TGIF2 monoclonal antibody (ab155948) and mouse anti-bovine vimentin monoclonal antibody (ab8978) were purchased from abcam (Cambridge, UK). The secondary antibodies; Horseradish peroxidase (HRP)-conjugated polymer anti-rabbit and anti-mouse antibodies were purchased from DAKO-Agilent Technologies Co. (Santa Clara, CA, USA). HRP-linked anti-rabbit and -mouse antibodies were purchased from Cell Signaling Technology Inc. (Danvers, MA, USA). Alexa Fluor 594-conjugated goat anti-rabbit IgG and Alexa Fluor 488-conjugated goat anti-mouse IgG antibodies were purchased from Invitrogen-Thermo Fisher Scientific (Waltham, MA, USA).