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Sto 609 acetate

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STO-609 acetate is a laboratory reagent used in biochemical research. It is a small molecule inhibitor that acts on specific cellular enzymes. The core function of STO-609 acetate is to serve as a research tool for investigating cellular signaling pathways and related biological processes.

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Lab products found in correlation

Rhoa inhibitor 1, by Cytoskeleton (1 mentions) Fk506, by Cayman Chemical (1 mentions) Bapta, by Cayman Chemical (1 mentions) Ck 666, by Cayman Chemical (1 mentions) A23187, by Cayman Chemical (1 mentions) Bms 5, by Cayman Chemical (1 mentions) Blebbistatin, by Cayman Chemical (1 mentions) Y 27632, by Cayman Chemical (1 mentions) G 6983, by Selleck Chemicals (1 mentions) Metformin, by Cayman Chemical (1 mentions) Anti rabbit hrp linked antibody, by Cell Signaling Technology (1 mentions) Anti mouse hrp linked antibody, by Cell Signaling Technology (1 mentions) Cholesterol, by Merck Group (1 mentions) Pd98059, by Enzo Life Sciences (1 mentions) Jasplakinolide, by Cayman Chemical (1 mentions) Methyl β cyclodextrin, by Merck Group (1 mentions) Cep 1347, by Bio-Techne (1 mentions) Cytochalasin d, by Cayman Chemical (1 mentions) Phospho jnk1 2, by Cell Signaling Technology (1 mentions) Total jnk1 2, by Cell Signaling Technology (1 mentions)

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STO-609 (21 citations)

5 protocols using sto 609 acetate

1

Pharmacological Regulation of Cell Migration

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MEF cells were treated with 1% DMSO, 20 µM BAPTA (Cayman Chemical), Y27632 5 µM (Cayman Chemical), 50 µM CK666 (Cayman Chemical), STO-609 acetate (Biotechne, #1551), A23187 (Cayman Chemical), 4 µM Blebbistatin (Cayman Chemical), 40 uM AIP (R&D Systems #5959/1), 50 µM CAS 1090893 (Millipore, #553511), 0.1 µg/ml RhoA inhibitor-I (Cytoskeleton, Inc, #CT-04), 2 µM FK-506 (Cayman Chemical, # 10007965), 300 nM FAK14 (Cayman Chemical, #14485), 10 µM BMS-5 (Cayman Chemical, #21072), or 2 µM ML-7 (Cayman Chemical, #11801) overnight during time-lapse imaging for random cell migration or followed by immunostaining.
Wang Y., Troughton L.D., Xu F., Chatterjee A., Ding C., Zhao H., Cifuentes L.P., Wagner R.B., Wang T., Tan S., Chen J., Li L., Umulis D., Kuang S., Suter D.M., Yuan C., Chan D., Huang F., Oakes P.W, & Deng Q. (2023). Atypical peripheral actin band formation via overactivation of RhoA and nonmuscle myosin II in mitofusin 2-deficient cells. eLife, 12, e88828.
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2

Pharmacological Reagents for Cell Signaling

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Reagents used throughout this study were obtained from the following sources: PD 102807, and STO-609 acetate, (Bio-Techne), acetyl-β-methylcholine chloride, and 5z-7-oxozealenol (Millipore), Gö 6983 (Selleck Chemicals), PKC-iota inhibitor 1 (compound 19) (MedChemExpress), and metformin (Cayman Chem). All other reagents described in the study were purchased from Sigma, ThermoFisher, or sources described previously (66 (link)).
Tompkins E., Mimic B., Penn R.B, & Pera T. (2023). The biased M3 mAChR ligand PD 102807 mediates qualitatively distinct signaling to regulate airway smooth muscle phenotype. The Journal of Biological Chemistry, 299(10), 105209.
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3

Molecular Signaling Pathway Analysis

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Cholesterol and methyl-β-cyclodextrin were purchased from Sigma-Aldrich. Compound C, STO-609 acetate and CEP-1347 were purchased from Tocris Bioscience. Cytochalasin D, Jasplakinolide and Latrunculin B were purchased from Cayman Chemical. PD98059 was purchased from Enzo Life Sciences. SP600125 and U0126 were purchased from Calbiochem. Antibodies for phospho-AMPKα (#2535), MyD88 (#4283), phospho-ERK1/2 (#9101), total-ERK1/2 (#9102), phospho-JNK1/2 (#9251), total-JNK1/2 (#9252), anti-rabbit HRP-linked antibody (#7074) and anti-mouse HRP-linked antibody (#7076) were purchased from Cell Signaling Technology. Antibodies for AMPKα1 (sc-19128), MUC5AC (sc-21701), TAK1 (sc-7967), MLK3 (sc-166592), c-myc (sc-40), β-actin (sc-8432) and donkey anti-goat HRP-linked antibody (sc-2020) were purchased from Santa Cruz Biotechnology. Donkey anti-rabbit IgG Alexa Fluor 488 (A-21206) and goat anti-mouse IgG Alexa Fluor 546 (A-11030) was purchased from Thermo Fisher Scientific. The human validated short interfering RNA (siRNA) oligonucleotides for AMPKα1 (M-005027-02-0005), TAK1 (M-003790-06-0005) and control siRNA (D-001206-14-05) were purchased from Dharmacon.
Matsuyama S., Komatsu K., Lee B.C., Tasaki Y., Miyata M., Xu H., Shuto T., Kai H, & Li J.D. (2022). Negative cross-talk between TLR2/4-independent AMPKα1 and TLR2/4-dependent JNK regulates S. pneumoniae-induced mucosal innate immune response. Journal of immunology (Baltimore, Md. : 1950), 209(8), 1532-1544.
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4

AMPK Signaling Pathway Modulators Protocol

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Phenylephrine hydrochloride, serotonin (5-hydroxytryptamine hydrochloride), and acetylcholine chloride were purchased from Sigma-Aldrich (St. Louis, MO). Metformin hydrochloride, AICAR (5-aminoimidazole-4-carboxamide-1-β-D-ribofuranoside or acadesine), compound C (or dorsomorphin dihydrochloride), and STO-609 acetate were purchased from Tocris Bioscience (Minneapolis, MN). Phenformin hydrochloride and L-NMMA (L-NG-monomethyl arginine acetate) were purchased from Cayman Chemical (Ann Arbor, MI). Adenosine 5’-triphosphate disodium (ATP), adenosine 5’-diphosphate monosodium (ADP), and adenosine 5’-monophosphate disodium (AMP) were purchased from EMD Millipore Chemicals (Billerica, MA). The primary antibodies for phospho-AMPKαThr172 (2535) and AMPKα (2532) were purchased from Cell Signaling Technology (Danvers, MA). The primary antibody for CaMKKβ (sc-50341) was purchased from Santa Cruz Biotechnology (Santa Cruz, CA). The primary antibody for β-actin was purchased from Abcam (Cambridge, MA). HRP-conjugated goat anti-rabbit secondary antibody was from Bio-Rad (Hercules, CA). All other chemicals were from Fisher Scientific (Fair Lawn, NJ) or Sigma-Aldrich (St. Louis, MO).
Pyla R., Osman I., Pichavaram P., Hansen P, & Segar L. (2014). Metformin exaggerates phenylephrine-induced AMPK phosphorylation independent of CaMKKβ and attenuates contractile response in endothelium-denuded rat aorta. Biochemical pharmacology, 92(2), 266-279.
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5

Electrophysiological Profiling of Ion Channels

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Chemical reagents used included tetrodotoxin, bicuculline, DL-2-amino-5-phosphonopentanoic, Nifedipine, STO-609 acetate, XE991 dihydrochloride (all Tocris), and dendrotoxin-K (Alomone). Antibodies used included anti-Lgi1 (Abcam), anti-GAPDH (Cell Signaling), anti-Kv1.1 (Millipore), anti-Kv1.1, anti-Kv1.4 (Neuromab), and anti-KCNQ3 (Alamone, or a kind gift from Dr. E. Cooper, Baylor College of Medicine, Houston, Texas).
Lee K.Y., Royston S.E., Vest M.O., Ley D.J., Lee S., Bolton E.C, & Chung H.J. (2015). N-methyl-D-aspartate receptors mediate activity-dependent down-regulation of potassium channel genes during the expression of homeostatic intrinsic plasticity. Molecular Brain, 8, 4.
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