Annexin 5 fitc conjugate

Manufactured by Merck Group

Annexin V/FITC conjugate is a fluorescent labeling reagent used for the detection and quantification of apoptosis (programmed cell death) in various cell types. Annexin V binds to phosphatidylserine, which is exposed on the cell surface during apoptosis. The FITC (fluorescein isothiocyanate) label allows for the visualization and analysis of apoptotic cells using flow cytometry or fluorescence microscopy.

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Lab products found in correlation

Propidium iodide, by Merck Group (2 mentions) Colorimetric assay kit, by Cayman Chemical (1 mentions) Binding buffer, by Merck Group (1 mentions) Annexin 5 fitc, by Cayman Chemical (1 mentions) Phosphate buffered saline (pbs), by Corning (1 mentions) Trypsin 2.21 mm edta solution, by Corning (1 mentions) Propidium iodide solution, by Merck Group (1 mentions)

Spelling variants of this product

Annexin V-FITC (217 citations) Annexin V (119 citations) FITC conjugate (7 citations)

3 protocols using annexin 5 fitc conjugate

Annexin V/PI Assay for Zirconia Nanoparticles

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HaCaT cells were cultured and exposed to yttria-stabilized zirconia nanoparticles for 24 and 48 hrs. After exposure, the cells were fixed with paraformaldehyde (4%) for 5 mins. The fixed cells were washed in chilled PBS and stained with a mixture of annexin V/FITC conjugate (2 µL) and propidium iodide (10 µL/mL) prepared in binding buffer (500 µL) (Sigma). The fluorescent image was examined under a confocal microscope with excitation 488 nm and emission 520 nm for annexin V/FITC and excitation 536 nm and emission 617 nm for propidium iodide.28 (link)
The caspase-3 enzyme was determined in HaCaT cells after exposure to yttria-stabilized zirconia nanoparticles for 24 and 48 hrs by using Cayman Chemical colorimetric assay kits.

Alzahrani F.M., Katubi K.M., Ali D, & Alarifi S. (2019). Apoptotic and DNA-damaging effects of yttria-stabilized zirconia nanoparticles on human skin epithelial cells. International Journal of Nanomedicine, 14, 7003-7016.

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Annexin V and Propidium Iodide Apoptosis Assay

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Cells with seed density of 5 × 10⁵ will be added to T-25 vented flask in phenol-free IMEM supplemented medium and allowed to grow for 70–80% confluency. The cells were then treated with 1 μM test peptide and/or 2 nM E2 for 16 h or 24 h and incubated at 37 °C with 5% CO₂ and 10% humidity. The floating cells from each condition were stored in conical tubes and the attached cells were washed with PBS (Corning) and removed by incubating them with 0.25% trypsin 2.21 mM EDTA solution (Corning). Trypsinized and removed from the cells by centrifuging for 5 min at 1000 RPM and aspirating the supernatant. The cells were resuspended in binding buffer (10 mM HEPES/NaOH pH7.5 containing 0.14 M NaCl and 2.5 mM CaCl₂) and transferred to test tubes. Annexin V FITC Conjugate (1 μg/ml) (Sigma) and Propidium Iodide (1 μg/ml) (Sigma) were added to all tubes, including an untreated control, except in the untreated, unstained control. The cells were incubated with the dyes for 10 min in the dark. Flow cytometry was performed using a Becton Dickinson Fortessa SORP 14-color flow cytometer equipped with a 488 nm excitation filter, a 515/20 nm emission filter and a 610/20 nm emission filter.

Chakraborty S., Asare B.K., Biswas P.K, & Rajnarayanan R.V. (2016). Designer interface peptide grafts target estrogen receptor alpha dimerization. Biochemical and biophysical research communications, 478(1), 116-122.

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Annexin V-FITC and PI Apoptosis Assay

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Cells were digested, and washed twice with 1× PBS, then resuspended in binding buffer to a concentration of 1 × 10⁶ cells/mL. Cell suspensions (500 µL) were added to tubes, then added 5 uL of Annexin V FITC conjugate and 10 µL of Propidium Iodide Solution (both available from Sigma-Aldrich) and incubated at room temperature for 10 min and protected from light. Fluorescence of the cells were analyzed by flow cytometry.

Liu X., Tian H., Li H., Ge C., Zhao F., Yao M, & Li J. (2017). Derivate Isocorydine (d-ICD) Suppresses Migration and Invasion of Hepatocellular Carcinoma Cell by Downregulating ITGA1 Expression. International Journal of Molecular Sciences, 18(3), 514.

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