Vented culture flasks

Manufactured by Corning

Sourced in United States

Vented culture flasks are a type of lab equipment designed for the growth and maintenance of cell cultures. They feature a vented cap that allows for gas exchange, which is essential for cell respiration and culture health.

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Lab products found in correlation

Fetal bovine serum (fbs), by Thermo Fisher Scientific (2 mentions) Trypan blue, by Thermo Fisher Scientific (1 mentions) Amphotericin b, by Thermo Fisher Scientific (1 mentions)

Close spelling variants of this product

25 cm2 vented culture flasks Vented 75 cm2 tissue culture flasks Vented flasks Culture flasks

4 protocols using vented culture flasks

Culturing Bloodstream T. brucei

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Bloodstream forms of T. brucei Lister 427 were cultured
in Hirumi-9 (HMI-9) medium (Invitrogen), supplemented with 10% heat
inactivated fetal bovine serum (FBS; Gibco) in vented culture flasks
(Corning), at 37 °C, in a 5% CO₂ atmosphere, as described
previously.^{46 (link)}

Blaazer A.R., Singh A.K., de Heuvel E., Edink E., Orrling K.M., Veerman J.J., van den Bergh T., Jansen C., Balasubramaniam E., Mooij W.J., Custers H., Sijm M., Tagoe D.N., Kalejaiye T.D., Munday J.C., Tenor H., Matheeussen A., Wijtmans M., Siderius M., de Graaf C., Maes L., de Koning H.P., Bailey D.S., Sterk G.J., de Esch I.J., Brown D.G, & Leurs R. (2018). Targeting a Subpocket in Trypanosoma brucei Phosphodiesterase B1 (TbrPDEB1) Enables the Structure-Based Discovery of Selective Inhibitors with Trypanocidal Activity. Journal of Medicinal Chemistry, 61(9), 3870-3888.

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Cytotoxicity Assay for Drug Screening

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All cell lines were cultured in monolayers in sterile, vented culture flasks (Corning). Cells were passaged, collected, and then counted with 0.4% Trypan Blue (Thermo Fisher Scientific). Cells were then formulated in their respective media and plated in 96-well plates at 5,000 cells per well in 100 μL. After allowing cells to incubate at 37°C for 12 h, drugs were then added to the wells. Each drug was formulated at the highest concentration in the cell media and then serially diluted. A PTX equivalent concentration between 10⁻⁴ and 10⁻¹² M of each construct was plated in quadruplicate and allowed to incubate for 72 h. 20 μL of CellTiter 96^® AQ_ueoous One solution (Promega) was then added to each well. After 1.5 h, the absorbance of each plate was read at 490 nm and normalized to untreated cells to determine the relative cell survival. The half effective concentration (EC₅₀) was calculated as the inflection between the curve’s maxima and minima. The IC₅₀, or half maximal inhibitory concentration, was measured at the point where cell survival was equal to 50% of the cell survival (Fig. S20).

Michalkova-Papajova D., Obernauerova M, & Subik J. (2000). Role of the PDR Gene Network in Yeast Susceptibility to the Antifungal Antibiotic Mucidin. Antimicrobial Agents and Chemotherapy, 44(2), 418-420.

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Isolation and Culture of Antarctic Snow Algae

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A total of two genera of snow algae both isolated from King George Island, Antarctica and Spitsbergen, Svalbard were investigated during the present study (Table 1). Clonal algal cultures were acquired from the Culture Collection of Cryophilic Algae (CCCryo) at the Fraunhofer-IBMT in Potsdam (see Table 1 for strain numbers and origin). Cultures were transported to the Low Temperature Experimental Facility (LOWTEX) at the University of Bristol and stored in vented culture flasks (Corning, New York, USA) with nutrient replete 3N-BBM prior to experimentation (Bischoff and Bold 1963 , Andersen 2005 ).

Broadwell E.L., Pickford R.E., Perkins R.G., Sgouridis F, & Williamson C.J. (2023). Adaptation versus plastic responses to temperature, light, and nitrate availability in cultured snow algal strains. FEMS Microbiology Ecology, 99(9), fiad088.

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Cultivation of HeLa and MCF7 Cell Lines

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The in vitro studies were carried out on cervical and breast cancer cell line HeLa and MCF7 respectively, obtained from National Centre for Cell Science, Pune, India. The cells were grown on 25 cm² vented culture flasks (Corning, USA) using Eagle's Minimal Essential Medium (EMEM) (Thermofisher, USA) supplemented with 10% heat-inactivated fetal bovine serum (Thermofisher, USA), and 100 U/mL Penicillin, 100 µg/mL Streptomycin and 0.25 µg/mL Amphotericin B (Thermofisher, USA), in a 37 °C incubator with 5% CO₂ and 95% relative humidity.

Bhardwaj A., Parekh K, & Jain N. (2020). In vitro hyperthermic effect of magnetic fluid on cervical and breast cancer cells. Scientific Reports, 10, 15249.

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