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H3k4me2 c75h12

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H3K4me2 (C75H112N20O18) is a histone modification-specific antibody that recognizes the dimethylation of lysine 4 on histone H3. This modification is associated with active transcription and is commonly used as a marker for actively transcribed chromatin regions.

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Lab products found in correlation

Ac 74, by Merck Group (2 mentions) Nupage transfer buffer, by Thermo Fisher Scientific (2 mentions) Nupage bis tris polyacrylamide gel, by Thermo Fisher Scientific (2 mentions) Supersignal west pico, by Thermo Fisher Scientific (2 mentions) Lds loading buffer, by Thermo Fisher Scientific (2 mentions) H3k36me2 c64g9, by Cell Signaling Technology (2 mentions) Supersignal west femto chemiluminescence kit, by Thermo Fisher Scientific (2 mentions) H3k27me3 c36b11, by Cell Signaling Technology (2 mentions) Supersignal western blot enhancer kit, by Thermo Fisher Scientific (2 mentions) 0.2 μm pvdf membrane, by Bio-Rad (2 mentions) H3k4me3, by Cell Signaling Technology (2 mentions)

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H3K4me2 (32 citations)

2 protocols using h3k4me2 c75h12

1

Quantifying Histone Modifications by Western Blot

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Equal numbers of cells from each population were sorted into trichloroacetic acid (TCA, Sigma), and the TCA final concentration was adjusted to 10%. Samples were centrifuged at 16,000g for 15 minutes, and precipitates were washed twice with cold acetone and dried. Samples were solubilized in 9M urea, 2% Triton X-100, 1% DTT. LDS loading buffer (Life Technologies) was added. Samples were heated at 70°C for 10 minutes, separated on NuPAGE Bis-Tris polyacrylamide gels (Life Technologies), and transferred to 0.2μm PVDF membranes (BioRad) by wet transfer using NuPAGE transfer buffer (Life Technologies). Western blots were performed using antibodies against β-actin (AC-74, Sigma), histone H3 (polyclonal, Abcam, ab1791), H3K4me3 (C42D8, Cell Signaling), H3K27me3 (C36B11, Cell Signaling), H3K4me2 (C75H12, Cell Signaling) and H3K36me2 (C64G9, Cell Signaling). For small cell numbers (7,000-15,000), the SuperSignal Western Blot Enhancer kit (Thermo Scientific) was used according to the manufacturer instructions. Signals were detected using the SuperSignal West Pico or SuperSignal West Femto chemiluminescence kits (Thermo Scientific). Blots were stripped with 0.2N NaOH and/or 1% SDS, 25mM glycine, pH=2 before re-probing.
Agathocleous M., Meacham C.E., Burgess R.J., Piskounova E., Zhao Z., Crane G.M., Cowin B.L., Bruner E., Murphy M.M., Chen W., Spangrude G.J., Hu Z., DeBerardinis R.J, & Morrison S.J. (2017). Ascorbate regulates haematopoietic stem cell function and leukaemogenesis. Nature, 549(7673), 476-481.
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2

Quantifying Histone Modifications by Western Blot

Check if the same lab product or an alternative is used in the 5 most similar protocols
Equal numbers of cells from each population were sorted into trichloroacetic acid (TCA, Sigma), and the TCA final concentration was adjusted to 10%. Samples were centrifuged at 16,000g for 15 minutes, and precipitates were washed twice with cold acetone and dried. Samples were solubilized in 9M urea, 2% Triton X-100, 1% DTT. LDS loading buffer (Life Technologies) was added. Samples were heated at 70°C for 10 minutes, separated on NuPAGE Bis-Tris polyacrylamide gels (Life Technologies), and transferred to 0.2μm PVDF membranes (BioRad) by wet transfer using NuPAGE transfer buffer (Life Technologies). Western blots were performed using antibodies against β-actin (AC-74, Sigma), histone H3 (polyclonal, Abcam, ab1791), H3K4me3 (C42D8, Cell Signaling), H3K27me3 (C36B11, Cell Signaling), H3K4me2 (C75H12, Cell Signaling) and H3K36me2 (C64G9, Cell Signaling). For small cell numbers (7,000-15,000), the SuperSignal Western Blot Enhancer kit (Thermo Scientific) was used according to the manufacturer instructions. Signals were detected using the SuperSignal West Pico or SuperSignal West Femto chemiluminescence kits (Thermo Scientific). Blots were stripped with 0.2N NaOH and/or 1% SDS, 25mM glycine, pH=2 before re-probing.
Agathocleous M., Meacham C.E., Burgess R.J., Piskounova E., Zhao Z., Crane G.M., Cowin B.L., Bruner E., Murphy M.M., Chen W., Spangrude G.J., Hu Z., DeBerardinis R.J, & Morrison S.J. (2017). Ascorbate regulates haematopoietic stem cell function and leukaemogenesis. Nature, 549(7673), 476-481.
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