Recombinant human insulin solution (10 mg/mL in 25 mM hydroxyethyl piperazineethanesulfonic acid (HEPES) at pH 8.2; activity, 27 units/mg), fluorescein-5-isothiocyanate (FITC), dimethylsulfoxide, streptozotocin, and chitosan (low molecular weight, 50–190 kDa, 75% deacetylation) were obtained from Sigma-Aldrich (Poole, UK). Heparin sodium salt was provided by AppliChem (Darmstadt, Germany). Poly(ethylene) glycol (PEG) 6000 was purchased from BDH Laboratory Supplies (Poole, UK).
Recombinant human insulin solution
Manufactured by Merck Group
Sourced in United Kingdom, Switzerland
Recombinant human insulin solution is a laboratory product that contains a synthetic form of human insulin. It is produced using recombinant DNA technology. The solution provides a standardized source of insulin for research and development purposes.
Automatically generated - may contain errors
Lab products found in correlation
Fluorescein 5 isothiocyanate fitc, by Merck Group (1 mentions)
Heparin sodium salt, by AppliChem (1 mentions)
Chitosan, by Merck Group (1 mentions)
Dimethyl sulfoxide (dmso), by Merck Group (1 mentions)
Streptozotocin (stz), by Merck Group (1 mentions)
Rabbit anti ulk1, by Cell Signaling Technology (1 mentions)
Rabbit anti igf1rβ, by Cell Signaling Technology (1 mentions)
Rabbit anti ir β, by Santa Cruz Biotechnology (1 mentions)
Recombinant human igf 1, by Thermo Fisher Scientific (1 mentions)
Mouse anti gfap, by Merck Group (1 mentions)
Rabbit anti p62, by Cell Signaling Technology (1 mentions)
Rabbit anti iba1, by Fujifilm (1 mentions)
Rabbit anti gapdh, by Cell Signaling Technology (1 mentions)
Rabbit anti lc3a b, by Cell Signaling Technology (1 mentions)
Bafilomycin a1, by Cell Signaling Technology (1 mentions)
Glutamax, by Thermo Fisher Scientific (1 mentions)
Penicillin streptomycin p s, by Thermo Fisher Scientific (1 mentions)
Hepes, by Lonza (1 mentions)
3 protocols using recombinant human insulin solution
1
Insulin-Based Diabetic Model Development
2
Adenovirus-Mediated Protein Flux Analysis
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Adenovirus encoding CMV promoter-driven GFP:Cre or GFP alone were purchased from Vector Biolabs. Adenovirus encoding mRFP-GFP tandem fluorescent-tagged LC3 (tfLC3) reporter was kindly provided by Dr. Qiangrong Liang (23). Recombinant human insulin solution was purchased from Sigma, whereas recombinant human IGF-1 was from PeproTech. Bafilomycin A1 was from Cell Signaling Technology. Rabbit anti-p62 (#23214), rabbit anti-LC3A/B (#12741), rabbit anti-Ulk1 (#8054), rabbit anti-IGF1Rβ (#9750), rabbit anti-GAPDH (#5174) were purchased from Cell Signaling Technology. Rabbit anti-IRβ (#sc-711) was from Santa Cruz. Mouse anti-GFAP (#MAB306) and mouse anti-NeuN (#MAB377B) were purchased from Millipore. Rabbit anti-GFAP (#ab7260) was purchased from Abcam. Rabbit anti-Iba-1 (#019-19741) was purchased from Fujifilm-Wako. HRP-conjugated goat-anti-rabbit IgG secondary antibodies were purchased from GE Healthcare.
3
PCP-R Cell Culture Protocol
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PCP-R cell culture was performed as described previously [13] , with the following modifications. The cells were cultivated in DMEM containing 4.5 gl -1 glucose, pyruvate, and phenol red (GlutaMAX™, Gibco by Life Technologies, Carlsbad, USA) and supplemented with 10% (v/v) fetal calf serum (FCS; Gibco by Life Technologies, Carlsbad, USA), 2% (v/v) Penicillin-Streptomycin (P/S; 5000 U ml -1 stock; Gibco by Life Technologies, Carlsbad, USA), 2.4% (v/v) HEPES (1 M stock; Lonza, Basel, Switzerland), 0.05% (v/v) recombinant human insulin solution (10 mg ml -1 stock; Sigma-Aldrich, St. Louis, MO, USA). This medium composition will be referred to as 10% FCS PCP-R medium.
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