To prepare bone marrow-derived macrophages (BMDMs), bone marrow cells from WT and Tyk2 KO mice were cultured in RPMI supplemented with 10% FBS and recombinant murine macrophage colony stimulating factor (100 ng/ml; PeproTech, Rocky Hill, NJ) for 6 days. BMDMs and peritoneal resident cells collected from the peritoneal lavage were plated in RPMI-1640 medium supplemented with 10% FBS, 100 U/ml penicillin and 0.1 mg/ml streptomycin. IL-10 levels in the culture supernatants of BMDMs or peritoneal resident cells were determined using an IL-10 ELISA kit (eBioscience). The following agents were tested for their effects on IL-10 production: diclofenac sodium (10 µM; Wako Pure Chemical Industries, Osaka, Japan), and H-89 (10 µM; Cell Signaling Technologies, Beverly, MA).
Diclofenac sodium
Manufactured by Fujifilm
Sourced in Japan
Diclofenac sodium is a laboratory product used as a reference standard. It is a white or almost white, crystalline powder. Diclofenac sodium is a non-steroidal anti-inflammatory drug (NSAID) often used for research and development purposes.
Automatically generated - may contain errors
Lab products found in correlation
Il 10 elisa kit, by Thermo Fisher Scientific (1 mentions)
Recombinant murine macrophage colony stimulating factor, by Thermo Fisher Scientific (1 mentions)
Penicillin streptomycin, by Thermo Fisher Scientific (1 mentions)
Dulbecco s phosphate buffered saline dpbs, by Thermo Fisher Scientific (1 mentions)
Bovine serum albumin (bsa), by Merck Group (1 mentions)
Hyaluronidase, by Seikagaku (1 mentions)
Fetal bovine serum (fbs), by MP Biomedicals (1 mentions)
Ohpak sb 805 hq, by Resonac (1 mentions)
Il 1β, by R&D Systems (1 mentions)
Lidocaine, by Tokyo Chemical Industry (1 mentions)
Aminopyrine, by Fujifilm (1 mentions)
Pilocarpine hydrochloride, by Merck Group (1 mentions)
Tranilast, by Tokyo Chemical Industry (1 mentions)
Antipyrine, by Fujifilm (1 mentions)
Rhodamine b, by Fujifilm (1 mentions)
Heparin sodium, by Fujifilm (1 mentions)
Lps from escherichia coli o127 b8, by Merck Group (1 mentions)
5 protocols using diclofenac sodium
1
Murine Bone Marrow-Derived Macrophage Isolation
2
Synoviocyte Metabolism Modulation
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ARTZ Dispo® (1% HA) and hyaluronidase were purchased from Seikagaku Corporation (Tokyo, Japan). Diclofenac sodium was from Wako Pure Chemical Industries, Ltd. (Osaka, Japan). Water for injection (WFI) was from Otsuka Pharmaceutical Factory, Inc. (Tokushima, Japan). Human fibroblast-like synoviocytes from rheumatoid arthritis (HFLS-RA) patients and those from osteoarthritis (HFLS-OA) patients were purchased from Cell Applications, Inc. (San Diego, CA). Growth medium and a subculture reagent kit were from Cell Applications, Inc. α-MEM, α-MEM powder, penicillin/streptomycin and Dulbecco’s phosphate buffered saline (D-PBS) were from Life Technologies Corporation (Waltham, MA). FBS was from MP Biomedicals LLC. (Santa Ana, CA). IL-1β was from R&D Systems, Inc. (Minneapolis, MN). Bovine serum albumin (BSA) was from Sigma-Aldrich Co. LLC. (St. Louis, MO). Glucosamine hydrochloride, D-[6-3H (N)] ([3H]glucosamine) (37 MBq/mL) and Ultima-FloTMM (scintillation solution) were from PerkinElmer Co., Ltd. (Waltham, MA). OH pak SB-805 HQ and OH pak SB-807 HQ columns were from SHOWA DENKO K.K. (Tokyo, Japan). Select-HATM 500 k, Select-HATM 1000 k, and Select-HATM 2500 k were from Hyalose, LLC. (Oklahoma City, OK).
3
Fabrication of Microneedle Patch with Diclofenac
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Sodium hyaluronate (HA) (FCH-SU, lot: HGV016900, average molecular weight (MW): 50,000-110,000 Da) was purchased from Kikkoman Biochemifa Company (Tokyo, Japan). Diclofenac sodium (DCF) was purchased from FUJIFILM Wako Pure Chemical Corporation (Osaka, Japan).
Polydimethylsiloxane (PDMS, Dowsil Silpot 184) was purchased from Dow Corning (Midland, MI, USA). Aluminum microneedle mold masters, fabricated via micro-milling, were purchased from Tokai Azumi Techno (Mie, Japan). All other chemicals were purchased commercially as reagent-grade products. Water was deionized and purified using a Milli-Q® TOC purification system (Millipore, Bedford, MA, USA).
Polydimethylsiloxane (PDMS, Dowsil Silpot 184) was purchased from Dow Corning (Midland, MI, USA). Aluminum microneedle mold masters, fabricated via micro-milling, were purchased from Tokai Azumi Techno (Mie, Japan). All other chemicals were purchased commercially as reagent-grade products. Water was deionized and purified using a Milli-Q® TOC purification system (Millipore, Bedford, MA, USA).
4
Purchasing Chemicals for Research
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Pilocarpine hydrochloride was purchased from Sigma-Aldrich (St. Louis, MO, USA).
Lidocaine and tranilast were purchased from Tokyo Chemical Industry Co. Ltd. (Tokyo, Japan). Aminopyrine, antipyrine, diclofenac sodium, fluorescein sodium, and rhodamine B were purchased from Wako Pure Chemical Industries, Ltd. (Osaka, Japan).
Lidocaine and tranilast were purchased from Tokyo Chemical Industry Co. Ltd. (Tokyo, Japan). Aminopyrine, antipyrine, diclofenac sodium, fluorescein sodium, and rhodamine B were purchased from Wako Pure Chemical Industries, Ltd. (Osaka, Japan).
5
LPS-Induced Inflammatory Response
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LPS from Escherichia coli O127:B8 (lot number: 103V4051, 064M4021V, or 037M4067V) was purchased from Sigma-Aldrich, Inc. (St. Louis, MO, USA). The endotoxin content was > 500,000 endotoxin units (EU)/mg. Diclofenac sodium and heparin sodium were from FUJIFILM Wako Pure Chemical Industries (Osaka, Japan).
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